G-Protein-Coupled Receptor Kinase 2 Inhibition Induces Meiotic Arrest by Disturbing Ca 2+ Release in Porcine Oocytes.

Autor: Kim JD; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Lee SH; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Li XH; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Lu QY; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Zhan CL; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Lee GH; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Sim JM; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Song HJ; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Zhou D; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea., Cui XS; Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, South Korea.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2024 Sep; Vol. 59 (9), pp. e14715.
DOI: 10.1111/rda.14715
Abstrakt: G-protein-coupled receptor kinase 2 (GRK2) interacts with Gβγ and Gαq, subunits of G-protein alpha, to regulate cell signalling. The second messenger inositol trisphosphate, produced by activated Gαq, promotes calcium release from the endoplasmic reticulum (ER) and regulates maturation-promoting factor (MPF) activity. This study aimed to investigate the role of GRK2 in MPF activity during the meiotic maturation of porcine oocytes. A specific inhibitor of GRK2 (βi) was used in this study. The present study showed that GRK2 inhibition increased the percentage of oocyte arrest at the metaphase I (MI) stage (control: 13.84 ± 0.95%; βi: 31.30 ± 4.18%), which resulted in the reduction of the maturation rate (control: 80.36 ± 1.94%; βi: 65.40 ± 1.14%). The level of phospho-GRK2 decreased in the treated group, suggesting that GRK2 activity was reduced upon GRK2 inhibition. Furthermore, the addition of βi decreased Ca 2+ release from the ER. The protein levels of cyclin B and cyclin-dependent kinase 1 were higher in the treatment group than those in the control group, indicating that GRK2 inhibition prevented a decrease in MPF activity. Collectively, GRK2 inhibition induced meiotic arrest at the MI stage in porcine oocytes by preventing a decrease in MPF activity, suggesting that GRK2 is essential for oocyte meiotic maturation in pigs.
(© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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