Effect of different crystal forms of MnO 2 quenchers on the sensitivity of copper nanoclusters and their use in acidphosphatase activity.

Autor: Wen X; School of Chemistry and Materials Science, Shanxi Normal University, TaiYuan, 030032, China., Pang C; School of Chemistry and Materials Science, Shanxi Normal University, TaiYuan, 030032, China., Wang Y; School of Chemistry and Materials Science, Shanxi Normal University, TaiYuan, 030032, China., Li F; School of Chemistry and Materials Science, Shanxi Normal University, TaiYuan, 030032, China., Fan Z; School of Chemistry and Materials Science, Shanxi Normal University, TaiYuan, 030032, China. zhefengfan@126.com.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Sep 12; Vol. 191 (10), pp. 592. Date of Electronic Publication: 2024 Sep 12.
DOI: 10.1007/s00604-024-06660-2
Abstrakt: Highly stable copper nanocluster (CuNCs) with aggregation-induced emission (AIE) properties was synthesized. α-, β-, and γ- MnO 2 were utilized as quenchers, with CuNCs fluorescence quenching of 48.9%, 91.5%, and 96.6%, respectively. L-ascorbate-2-phosphate (AAP) was hydrolyzed by acid phosphatase (ACP), and ascorbic acid (AA) was formed. Then, MnO 2 could be restored by AA, and the fluorescence of the CuNCs could be restored. An on-off-on detection platform with a high signal/noise ratio was constructed for the sensing of ACP. The fluorescence recovery rate of the CuNCs was related to the crystal forms of MnO 2 . Then, the equilibrium constants (K) for the reaction between AA and MnO 2 were calculated to evaluate the reaction process. Compared with the K values of CuNCs/α-MnO 2 and CuNCs/γ-MnO 2 , the K values for AA and β-MnO 2 were maximum. The CuNCs/β-MnO 2 system exhibited optimal fluorescence recovery for the sensitive detection of ACP. In the concentration range 0.005-0.06 U/mL, the detection limit was 0.0028 U/mL. The determination  of serum ACP levels also revealed satisfactory results. This study provides novel insights into enhancing the sensitivity of the determination  of quenchers in different crystal form.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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