Population-based anti-Müllerian hormone reference intervals help define gonadal status in the bitch.

Autor:	Conley AJ; 1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA., Berger T; 2Department of Animal Science, College of Agricultural and Environmental Sciences, University of California-Davis, Davis, CA., Caruso CJ; 1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA., Cotterman RF; 1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA., Jones T; 1Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA., Christensen BW; 3Kokopelli Assisted Reproductive Services, Sacramento, CA., Hollinshead FK; 4Department of Clinical Sciences, College of Veterinary Medicine, Colorado State University, Fort Collins, CO., Place NJ; 5Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Jazyk:	angličtina
Zdroj:	Journal of the American Veterinary Medical Association [J Am Vet Med Assoc] 2024 Sep 11, pp. 1-8. Date of Electronic Publication: 2024 Sep 11.
DOI:	10.2460/javma.24.06.0405
Abstrakt:	Objective: To establish statistically valid, population-based reference intervals (RIs) for canine anti-Müllerian hormone (AMH) and define changes in AMH and inhibin-B in bitches during breeding cycles. Methods: A homologous canine ELISA was used to measure AMH in serum samples (collected between May 2019 and July 2024) from 102 intact and 78 reportedly ovariohysterectomized (OVH) bitches and 8 bitches before and after ovariohysterectomy, and in longitudinal samples from 24 bitches undergoing breeding management. Established 95% RIs were used in a retrospective assessment of 3,193 clinical submissions. Cyclic variation of AMH and inhibin-B (heterologous ELISA) were regressed with time and normalized to the rise in progesterone in samples from breeding bitches. Results: Intact and OVH RIs for AMH were calculated with and without inclusion of 7 samples from reportedly OVH bitches that had AMH concentrations in the intact RI. Anti-Müllerian hormone and inhibin-B were positively correlated, and AMH was 3 times higher in proestrus than in estrus. Retrospectively, of 3,193 samples submitted for clinical AMH testing, 41% to 56% were in or above the intact AMH interval, 37% to 44% were within the OVH interval, and < 10% were inconclusive, depending on how RIs were defined. Conclusions: Statistically valid, population-based RIs establish a sound basis for interpreting results of clinical submissions requesting AMH to assess gonadal status in the bitch. Clinical Relevance: Confirmation of cyclic variation in AMH (and, for the first time, inhibin-B) reaffirms proestrus as the optimum time to draw samples, and ≤ 10% of samples submitted for determination of gonadal status are expected to fall in an inconclusive AMH RI.
Databáze:	MEDLINE
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