In Vitro Anti-inflammatory Effects of Larch Turpentine, Turpentine Oil, Eucalyptus Oil, and Their Mixture as Contained in a Marketed Ointment.

Autor: Appel K; VivaCell Biotechnology GmbH, Denzlingen, Germany., Rose T; VivaCell Biotechnology GmbH, Denzlingen, Germany., Zimmermann C; Cesra Arzneimittel GmbH & Co. KG, Baden-Baden, Germany., Günnewich N; Cesra Arzneimittel GmbH & Co. KG, Baden-Baden, Germany.
Jazyk: angličtina
Zdroj: Planta medica [Planta Med] 2024 Oct; Vol. 90 (13), pp. 1023-1029. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1055/a-2388-7527
Abstrakt: An ointment containing larch turpentine, turpentine oil, and eucalyptus oil has been used for almost a century for the symptomatic treatment of mild, localized, purulent inflammations of the skin. Its clinical efficacy in the treatment of skin infections has been shown in clinical trials, but the mode of action of the active ingredients on inflammation is not known. We studied the anti-inflammatory properties of the active ingredients of the ointment and their mixture in a human monocyte cell model, in which the cells were stimulated with lipopolysaccharide and incubated with the test substances. The cytotoxic threshold of each test substance and the mixture was identified using the alamarBlue assay, and their anti-inflammatory activity was assessed by measuring the release of interleukins IL-1 β , IL-6, IL-8, monocyte chemoattractant protein-1, prostaglandin E 2 , and TNF- α . Cell toxicity was observed at a mixture concentration of 10 µg/mL. All immunological assays were carried out at nontoxic concentrations. Larch turpentine decreased IL-1 β , monocyte chemoattractant protein-1, and prostaglandin E 2 release at a concentration of 3.9 µg/mL and TNF- α at concentrations > 1.95 µg/mL, whereas eucalyptus oil and turpentine oil had no relevant inhibitory effects. The mixture dose-dependently inhibited IL-1 β , IL-6, monocyte chemoattractant protein-1, prostaglandin E 2 , and TNF- α release at concentrations > 1 µg/mL. IL-8 release was only marginally affected. The anti-inflammatory activity of the herbal ingredients and their mixture was confirmed in this model. This effect seems to be mediated mainly by larch turpentine, with turpentine oil and eucalyptus oil exerting an additive or possibly synergistic function.
Competing Interests: Christian Zimmermann and Nils Günnewich are employees of Cesra Arzneimittel GmbH. Kurt Appel and Thorsten Rose work for VivaCell Biotechnology GmbH, a contract research organization that specializes in preclinical research. All authors report no other conflicts of interest.
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Databáze: MEDLINE
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