Highly sensitive fluorescence detection of chloramphenicol based on product catalysis of tetrahedral DNA framework and fluorescent quenching of MIL-101(Fe).

Autor: Zou Y; State Key Laboratory of Macromolecular Drugs and Large-scale Manufacturing, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China., Wang W; College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China., Duan F; State Key Laboratory of Macromolecular Drugs and Large-scale Manufacturing, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China., Yun W; College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China., Chen H; Shanghai Institute of Doping Analyses, Shanghai University of Sport, Shanghai 200438, China. Electronic address: chenhong01@sus.edu.cn., Li Y; The Third Hospital of Mianyang/Sichuan Mental Health Center, Mianyang, Sichuan 621000, China. Electronic address: 13948076@qq.com., Yang L; State Key Laboratory of Macromolecular Drugs and Large-scale Manufacturing, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China. Electronic address: yanglz3000@aliyun.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2025 Jan 15; Vol. 463 (Pt 1), pp. 141144. Date of Electronic Publication: 2024 Sep 04.
DOI: 10.1016/j.foodchem.2024.141144
Abstrakt: An ultrasensitive fluorescence detection strategy of chloramphenicol (CAP) was developed based on product catalysis of tetrahedral DNA framework (TDF) and fluorescent quenching of MIL-101(Fe). The product was used to catalyze the reaction. As the concentration of catalyst increased, the reaction time was significantly shorted to 21 min which was much shorter than other isothermal amplification technologies. Moreover, the multiple fluorophores of TDF and high efficient quenching ability of MIL-101(Fe) provided better performance with a linear range for CAP detecting from 1.6 pM to 80 pM and the limit of detection (LOD) as low as 0.67 pM. In addition, it also demonstrated good specificity and resistance to interference from other related antibiotics. Importantly, this strategy exhibited satisfactory relative standard deviation and recovery results for practical application, exhibiting a favorable application prospect in CAP analysis.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Lizhu Yang reports financial support was provided by Wenzhou Science&Technology Bureau. Lizhu Yang reports financial support was provided by Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang. Yiping Li reports financial support was provided by Research Project of Mianyang Health Commission. Hong Chen reports financial support was provided by Research project of Shanghai University of Sport. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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