| Autor: |
Girão M; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal.; ICBAS - School of Medicine and Biomedical Sciences, University of Porto, Porto, Portugal., Lequint Z; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal.; Polytech Clermont, University Clermont Auvergne, Clermont-Ferrand, France., Rego A; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal., Costa I; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal., Proença DN; Department of Life Sciences, University of Coimbra, CEMMPRE, ARISE, Coimbra, Portugal., Morais PV; Department of Life Sciences, University of Coimbra, CEMMPRE, ARISE, Coimbra, Portugal., Carvalho MF; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal.; ICBAS - School of Medicine and Biomedical Sciences, University of Porto, Porto, Portugal. |
| Abstrakt: |
Two novel actinomycetal strains, designated CC-R113 T and CC-R104 T , were isolated from the tissues of two macroalgae collected on the northern Portuguese coast. Phylogenetic analyses based on the 16S rRNA gene showed that strain CT-R113 T belongs to the genus Nocardiopsis , being closely related to Nocardiopsis umidischolae 66/93 T and Nocardiopsis tropica VKM Ac-1457 T , with 98.65 and 98.39 % sequence similarity, respectively. The clade formed between the three type strains was confirmed by phylogenomic analysis. The genome of strain CT-R113 T was 7.27 Mb in size with a G+C content of 71.3 mol %, with average nucleotide identity (ANI) values of 89.59 and 90.14 % with strains 66/93 T and VKM Ac-1457 T , respectively. The major cellular fatty acids were identified as C 18 : 1 ω 9 c , iso-C 16 : 0 and anteiso-C 17 : 0 . Menaquinone 10 (MK-10) was the major respiratory quinone. Comparative analysis of 16S rRNA gene sequences showed that strain CC-R104 T belongs to the genus Rhodococcus and is most closely related to Rhodococcus pyridinivorans DSM 44555 T , with 98.24 % sequence similarity. However, phylogenomic analysis revealed that strain CC-R104 T establishes a clade with Rhodococcus artemisae DSM 45380 T , being more distant from Rhodococcus pyridinivorans DSM 44555 T . The genome of strain CC-R104 T was 5.34 Mb in size with a G+C content of 67.01 mol%. The ANI value between strains CC-R104 T and DSM 45380 T was 81.2 % and between strains CC-R104 T and DSM 44555 T was 81.5 %. The major cellular fatty acids were identified as C 18 : 1 ω 9 c , C 16 : 0 and summed feature 3. Menaquinone 8 (MK-8) was the only respiratory quinone. For both CC-R113 T and CC-R104 T , optimum growth was observed at pH 7.0, 28 °C and 0-5 % NaCl and whole-cell hydrolysates contained meso -diaminopimelic acid as the cell-wall diamino acid. On the basis of phenotypic, molecular and chemotaxonomic characteristics, strains CT-R113 T and CC-R104 T are considered to represent novel species, for which the names Nocardiopsis codii sp. nov. (type strain CT-R113 T =LMG33234 T =UCCCB172 T ) and Rhodococcus chondri sp. nov. (type strain CC-R104 T =LMG33233 T =UCCCB171 T ) are proposed. |
