Experimental viral spillover across 25 million year gap in Rodentia reveals limited viral transmission and purifying selection of a picornavirus.

Autor:	Shepherd FK; Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA., Roach SN; Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA., Sanders AE; Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA., Liu Y; Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA., Putri DS; Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA., Li R; Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA., Merrill N; Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA., Pierson MJ; Department of Lab Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA., Kotenko SV; Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers New Jersey Medical School, Newark, New Jersey, USA., Wang Z; Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA., Langlois RA; Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2024 Oct 16; Vol. 15 (10), pp. e0165024. Date of Electronic Publication: 2024 Sep 06.
DOI:	10.1128/mbio.01650-24
Abstrakt:	When a virus crosses from one host species to another, the consequences can be devastating. However, animal models to empirically evaluate cross-species transmission can fail to recapitulate natural transmission routes, physiologically relevant doses of pathogens, and population structures of naturally circulating viruses. Here, we present a new model of cross-species transmission where deer mice ( Peromyscus maniculatus ) are exposed to the natural virome of pet store mice ( Mus musculus ). Using RNA sequencing, we tracked viral transmission via fecal-oral routes and found the evidence of transmission of murine astroviruses, coronaviruses, and picornaviruses. Deep sequencing of murine kobuvirus revealed tight bottlenecks during transmission and purifying selection that leaves limited diversity present after transmission from Mus to Peromyscus . This work provides a structure for studying viral bottlenecks across species while keeping natural variation of viral populations intact and a high resolution look at within-host dynamics that occur during the initial stages of cross-species viral transmission.IMPORTANCEViral spillover events can have devastating public health consequences. Tracking cross-species transmission in real-time and evaluating viral evolution during the initial spillover event are useful for understanding how viruses adapt to new hosts. Using our new animal model and next generation sequencing, we develop a framework for understanding intrahost viral evolution and bottleneck events, which are very difficult to study in natural transmission settings. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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