Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens.

Autor:	Williams MR; School of Engineering & Technology, Institute for Great Lakes Research, Central Michigan University, Mt Pleasant, MI, USA., Telli AE; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey., Telli N; Department of Food Technology, Vocational School of Technical Sciences, Konya Technical University, Konya, Turkey., Islam DT; Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI, USA., Hashsham SA; Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI, USA. hashsham@egr.msu.edu.; Center for Microbial Ecology, Michigan State University, East Lansing, MI, USA. hashsham@egr.msu.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2852, pp. 3-17.
DOI:	10.1007/978-1-0716-4100-2_1
Abstrakt:	The use of direct nucleic acid amplification of pathogens from food matrices has the potential to reduce time to results over DNA extraction-based approaches as well as traditional culture-based approaches. Here we describe protocols for assay design and experiments for direct amplification of foodborne pathogens in food sample matrices using loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR). The examples provided include the detection of Escherichia coli in milk samples and Salmonella in pork meat samples. This protocol includes relevant reagents and methods including obtaining target sequences, assay design, sample processing, and amplification. These methods, though used for specific example matrices, could be applied to many other foodborne pathogens and sample types. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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