Feedstock variability impacts the bioconversion of sugar and lignin streams derived from corn stover by Clostridium tyrobutyricum and engineered Pseudomonas putida.
Autor: | Ruhl IA; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Nelson RS; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Katahira R; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Kruger JS; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Chen X; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Haugen SJ; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Ingraham MA; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Woodworth SP; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Alt H; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Ramirez KJ; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Peterson DJ; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Ding L; Energy and Environmental Science and Technology, Idaho National Laboratory, Idaho Falls, Idaho, USA., Laible PD; Biosciences Division, Argonne National Laboratory, Lemont, Illinois, USA., Linger JG; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA., Salvachúa D; Bioenergy Sciences and Technology Directorate, National Renewable Energy Laboratory, Golden, Colorado, USA. |
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Jazyk: | angličtina |
Zdroj: | Microbial biotechnology [Microb Biotechnol] 2024 Sep; Vol. 17 (9), pp. e70006. |
DOI: | 10.1111/1751-7915.70006 |
Abstrakt: | Feedstock variability represents a challenge in lignocellulosic biorefineries, as it can influence both lignocellulose deconstruction and microbial conversion processes for biofuels and biochemicals production. The impact of feedstock variability on microbial performance remains underexplored, and predictive tools for microbial behaviour are needed to mitigate risks in biorefinery scale-up. Here, twelve batches of corn stover were deconstructed via deacetylation, mechanical refining, and enzymatic hydrolysis to generate lignin-rich and sugar streams. These batches and their derived streams were characterised to identify their chemical components, and the streams were used as substrates for producing muconate and butyrate by engineered Pseudomonas putida and wildtype Clostridium tyrobutyricum, respectively. Bacterial performance (growth, product titers, yields, and productivities) differed among the batches, but no strong correlations were identified between feedstock composition and performance. To provide metabolic insights into the origin of these differences, we evaluated the effect of twenty-three isolated chemical components on these microbes, including three components in relevant bioprocess settings in bioreactors, and we found that growth-inhibitory concentrations were outside the ranges observed in the streams. Overall, this study generates a foundational dataset on P. putida and C. tyrobutyricum performance to enable future predictive models and underscores their resilience in effectively converting fluctuating lignocellulose-derived streams into bioproducts. (© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.) |
Databáze: | MEDLINE |
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