Lumpy skin disease virus isolation, experimental infection, and evaluation of disease development in a calf.

Autor: Adamu K; Virology Laboratory, Research and Development Directorate, National Veterinary Institute, P.O. Box 19, Bishoftu, Ethiopia. kassayeadamu881@gmail.com., Abayneh T; Bacteriology Laboratory, Research and Development Directorate, National Veterinary Institute, P.O. Box 19, Bishoftu, Ethiopia., Getachew B; Virology Laboratory, Research and Development Directorate, National Veterinary Institute, P.O. Box 19, Bishoftu, Ethiopia., Mohammed H; Virology Laboratory, Research and Development Directorate, National Veterinary Institute, P.O. Box 19, Bishoftu, Ethiopia., Deresse G; Molecular Biology Laboratory, Research and Development Directorate, National Veterinary Institute, P.O. Box 19, Bishoftu, Ethiopia., Zekarias M; Viral Vaccine Production Laboratory, Vaccine Production Directorate, National Veterinary Institute, P.O. Box 19, Bishoftu, Ethiopia., Chala W; Viral Vaccine Quality Control Laboratory, Quality Control Directorate, National Veterinary Institute, P.O. Box 19, Bishoftu, Ethiopia., Gelaye E; Food and Agriculture Organization of the United Nations, Sub-Regional Office for Eastern Africa, P.O. Box 5536, Addis Ababa, Ethiopia. Esayas.leykun@fao.org.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Sep 03; Vol. 14 (1), pp. 20460. Date of Electronic Publication: 2024 Sep 03.
DOI: 10.1038/s41598-024-60994-8
Abstrakt: Lumpy skin disease (LSD) is one of the most economically significant viral diseases of cattle caused by the Lumpy Skin Disease Virus (LSDV), classified as a member of the genus Capripoxvirus and belongs to the family Poxviridae. Nodular skin samples were collected from clinically sick cattle in the districts of Amuru and Wara Jarso Ethiopia to isolate LSD virus. The virus was isolated using primary lamb testis and kidney cells. The isolated LSDV was infected into a healthy calf while maintaining the necessary biosecurity measures to generate skin lesions and to assess disease progression using postmortem examinations. On the fourth day after virus inoculation, the calf developed typical LSD skin nodules with increased rectal temperature, which lasted until the 12th day, when they began to decrease. Viral shedding was detected in nasal, oral, and conjunctival swabs from 6 to 14 days after infection using real-time PCR. Post-mortem tissue specimens tested positive for LSD virus using real-time PCR and virus isolation. This study showed that LSDV were responsible for the LSD outbreaks, and the appearance of typical skin nodules accompanied by fever (> 39.5 °C) defined the virus's virulent status. The experimental infection with the isolated infectious LSDV could serve as a platform for future vaccine evaluation study using an LSDV challenge model.
(© 2024. The Author(s).)
Databáze: MEDLINE
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