| Autor: |
Xiao X; Department of Entomology, China Agricultural University, Beijing 100193, China., Yin XH; Department of Entomology, China Agricultural University, Beijing 100193, China., Hu SY; Department of Entomology, China Agricultural University, Beijing 100193, China., Miao HN; Department of Entomology, China Agricultural University, Beijing 100193, China., Wang Z; Department of Entomology, China Agricultural University, Beijing 100193, China.; Sanya Institute of China Agricultural University, Sanya 572024, China., Li H; Department of Entomology, China Agricultural University, Beijing 100193, China., Zhang YJ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Liang P; Department of Entomology, China Agricultural University, Beijing 100193, China., Gu SH; Department of Entomology, China Agricultural University, Beijing 100193, China.; Sanya Institute of China Agricultural University, Sanya 572024, China. |
| Abstrakt: |
The green peach aphid, Myzus persicae , is a worldwide agricultural pest. Chlorpyrifos has been widely used to control M. persicae for decades, thus leading to a high resistance to chlorpyrifos. Recent studies have found that insect odorant binding proteins (OBPs) play essential roles in insecticide resistance. However, the potential resistance mechanism underlying the cross-link between aphid OBPs and chlorpyrifos remains unclear. In this study, two OBPs (MperOBP3 and MperOBP7) were found overexpressed in M. persicae chlorpyrifos-resistant strains (CRR) compared to chlorpyrifos-sensitive strains (CSS); furthermore, chlorpyrifos can significantly induce the expression of both OBPs. An in vitro binding assay indicated that both OBPs strongly bind with chlorpyrifos; an in vivo RNAi and toxicity bioassay confirmed silencing either of the two OBPs can increase the susceptibility of aphids to chlorpyrifos, suggesting that overexpression of MperOBP3 and MperOBP7 contributes to the development of resistance of M. persicae to chlorpyrifos. Our findings provide novel insights into insect OBPs-mediated resistance mechanisms. |
