Effect of aging and exercise on hTERT expression in thymus tissue of hTERT transgenic bacterial artificial chromosome mice.

Autor: Kim JJ; School of Kinesiology, University of Michigan, Ann Arbor, MI, 48109, USA., Ahn A; School of Kinesiology, University of Michigan, Ann Arbor, MI, 48109, USA., Ying JY; School of Kinesiology, University of Michigan, Ann Arbor, MI, 48109, USA., Pollens-Voigt J; School of Kinesiology, University of Michigan, Ann Arbor, MI, 48109, USA., Ludlow AT; School of Kinesiology, University of Michigan, Ann Arbor, MI, 48109, USA. atludlow@umich.edu.
Jazyk: angličtina
Zdroj: GeroScience [Geroscience] 2024 Sep 02. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1007/s11357-024-01319-5
Abstrakt: Telomere shortening occurs with aging in immune cells and may be related to immunosenescence. Exercise can upregulate telomerase activity and attenuate telomere shortening in immune cells, but it is unknown if exercise impacts other immune tissues such as the thymus. This study aimed to examine human telomerase reverse transcriptase (hTERT) alternative splicing (AS) in response to aging and exercise in thymus tissue. Transgenic mice with a human TERT bacterial artificial chromosome integrated into its genome (hTERT-BAC) were utilized in two different exercise models. Mice of different ages were assigned to an exercise cage (running wheel) or not for 3 weeks prior to thymus tissue excision. Middle-aged mice (16 months) were exposed or not to treadmill running (30 min at 60% maximum speed) prior to thymus collection. hTERT transcript variants were measured by RT-PCR. hTERT transcripts decreased with aging (r =  - 0.7511, p < 0.0001) and 3 weeks of wheel running did not counteract this reduction. The ratio of exons 7/8 containing hTERT to total hTERT transcripts increased with aging (r = 0.3669, p = 0.0423) but 3 weeks of voluntary wheel running attenuated this aging-driven effect (r = 0.2013, p = 0.4719). Aging increased the expression of senescence marker p16 with no impact of wheel running. Thymus regeneration transcription factor, Foxn1, went down with age with no impact of wheel running exercise. Acute treadmill exercise did not induce any significant changes in thymus hTERT expression or AS variant ratio (p > 0.05). In summary, thymic hTERT expression is reduced with aging. Exercise counteracted a shift in hTERT AS ratio with age. Our data demonstrate that aging impacts telomerase expression and that exercise impacts dysregulated splicing that occurs with aging.
(© 2024. The Author(s), under exclusive licence to American Aging Association.)
Databáze: MEDLINE
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