Detection and molecular characterization of a novel mitovirus associated with Passiflora edulis Sims.

Autor: Santos YS; Centro de Educação e Saúde, Universidade Federal de Campina Grande, Cuité, PB, 58175-000, Brazil.; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770-917, Brazil.; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-900, Brazil., Vidal AH; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770-917, Brazil.; Instituto de Ciências Biológicas-IB, PPG BIOMOL, Universidade de Brasília, Brasília, DF, 70910-900, Brazil., Abreu EFM; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770-917, Brazil., Nogueira I; Instituto de Ciências Biológicas-IB, PPG BIOMOL, Universidade de Brasília, Brasília, DF, 70910-900, Brazil., Faleiro FG; Embrapa Cerrados, Distrito Federal, Planaltina, 73310-970, Brazil., Lacorte CC; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770-917, Brazil., Melo FL; Instituto de Ciências Biológicas-IB, PPG BIOMOL, Universidade de Brasília, Brasília, DF, 70910-900, Brazil., de Araújo Campos M; Centro de Educação e Saúde, Universidade Federal de Campina Grande, Cuité, PB, 58175-000, Brazil., de Rezende RR; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-900, Brazil., Morgan T; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-900, Brazil., Varsani A; The Bio design Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA., Alfenas-Zerbini P; Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-900, Brazil. palfenas@ufv.br., Ribeiro SG; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770-917, Brazil.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2024 Sep 02; Vol. 169 (9), pp. 190. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1007/s00705-024-06115-2
Abstrakt: Mitoviruses are cryptic capsidless viruses belonging to the family Mitoviridae that replicate and are maintained in the mitochondria of fungi. Complete mitovirus-like sequences were recently assembled from plant transcriptome data and plant leaf tissue samples. Passion fruit (Passiflora spp.) is an economically important crop for numerous tropical and subtropical countries worldwide, and many virus-induced diseases impact its production. From a large-scale genomic study targeting viruses infecting Passiflora spp. in Brazil, we detected a de novo-assembled contig with similarity to other plant-associated mitoviruses. The contig is ∼2.6 kb long, with a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRP). This contig has been named "passion fruit mitovirus-like 1" (PfMv1). An alignment of the predicted amino acid sequence of the RdRP of PfMv1 and those of other plant-associated mitoviruses revealed the presence of the six conserved motifs of mitovirus RdRPs. PfMv1 has 79% coverage and 50.14% identity to Humulus lupulus mitovirus 1. Phylogenetic analysis showed that PfMV1 clustered with other plant-associated mitoviruses in the genus Duamitovirus. Using RT-PCR, we detected a PfMv1-derived fragment, but no corresponding DNA was identified, thus excluding the possibility that this is an endogenized viral-like sequence. This is the first evidence of a replicating mitovirus associated with Passiflora edulis, and it should be classified as a member of a new species, for which we propose the name "Duamitovirus passiflorae".
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: