The C-terminal amino acid motifs of NS1 protein affect the replication and virulence of naturally NS-truncated H1N1 canine influenza virus.

Autor:	Wang P; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Guo J; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China., Zhou Y; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Zhu M; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Fang S; Department of Molecular Pharmacology, Tianjin Medical University Cancer Institute & Hospital, Tianjin, People's Republic of China.; National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Clinical Research Center for Cancer, Tianjin, People's Republic of China., Sun F; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Huang C; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Zhu Y; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Zhou H; Huabo Pet Hospital, Nanning, People's Republic of China., Pan B; Department of Molecular Pharmacology, Tianjin Medical University Cancer Institute & Hospital, Tianjin, People's Republic of China.; National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Clinical Research Center for Cancer, Tianjin, People's Republic of China., Qin Y; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Ouyang K; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Wei Z; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., Huang W; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China., García-Sastre A; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Global Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Chen Y; Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, People's Republic of China.; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, People's Republic of China.; Guangxi Key Laboratory of Animal Breeding, Disease Prevention and Control, Nanning, People's Republic of China.
Jazyk:	angličtina
Zdroj:	Emerging microbes & infections [Emerg Microbes Infect] 2024 Dec; Vol. 13 (1), pp. 2400546. Date of Electronic Publication: 2024 Sep 13.
DOI:	10.1080/22221751.2024.2400546
Abstrakt:	The vast majority of data obtained from sequence analysis of influenza A viruses (IAVs) have revealed that nonstructural 1 (NS1) proteins from H1N1 swine, H3N8 equine, H3N2 avian and the correspondent subtypes from dogs have a conserved four C-terminal amino acid motif when independent cross-species transmission occurs between these species. To test the influence of the C-terminal amino acid motifs of NS1 protein on the replication and virulence of IAVs, we systematically generated 7 recombinants, which carried naturally truncated NS1 proteins, and their last four C-terminal residues were replaced with PEQK and SEQK (for H1N1), EPEV and KPEI (for H3N8) and ESEV and ESEI (for H3N2) IAVs. Another recombinant was generated by removing the C-terminal residues by reverse genetics. Remarkably, the ESEI and KPEI motifs circulating in canines largely contributed efficient replication in cultured cells and these had enhanced virulence. In contrast, the avian ESEV motif was only responsible for high pathogenicity in mice. We examined the effects of these motifs upon interferon (IFN) induction. The 7 mutant viruses replicated in vitro in an IFN-independent manner, and the canine SEQK motif was able to induced higher levels of IFN-β in human cell lines. These findings shed further new light on the role of the four C-terminal residues in replication and virulence of IAVs and suggest that these motifs can modulate viral replication in a species-specific manner.
Databáze:	MEDLINE
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