Helical ultrastructure of the L-ENA spore aggregation factor of a Bacillus paranthracis foodborne outbreak strain.

Autor: Sleutel M; Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium. Mike.Sleutel@vub.be.; Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium. Mike.Sleutel@vub.be., Zegeye ED; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Llarena AK; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Pradhan B; Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.; Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium., Fislage M; Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.; Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium., O'Sullivan K; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Van Gerven N; Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.; Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium., Aspholm M; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway., Remaut H; Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium. Han.Remaut@vub.be.; Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium. Han.Remaut@vub.be.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Aug 29; Vol. 15 (1), pp. 7514. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1038/s41467-024-51804-w
Abstrakt: In pathogenic Bacillota, spores can form an infectious particle and can take up a central role in the environmental persistence and dissemination of disease. A poorly understood aspect of spore-mediated infection is the fibrous structures or 'endospore appendages' (ENAs) that have been seen to decorate the spores of pathogenic Bacilli and Clostridia. Current methodological approaches are opening a window on these long enigmatic structures. Using cryoID, Alphafold modelling and genetic approaches we identify a sub-class of robust ENAs in a Bacillus paranthracis foodborne outbreak strain. We demonstrate that L-ENA are encoded by a rare three-gene cluster (ena3) that contains all components for the self-assembly of ladder-like protein nanofibers of stacked heptameric rings, their anchoring to the exosporium, and their termination in a trimeric 'ruffle' made of a complement C1Q-like BclA paralogue. The role of ENA fibers in spore-spore interaction and the distribution of L-ENA operon as mobile genetic elements in B. cereus s.l. strains suggest that L-ENA fibers may increase the survival, spread and virulence of these strains.
(© 2024. The Author(s).)
Databáze: MEDLINE
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