Differential modulation of polycomb-associated histone marks by cBAF, pBAF, and gBAF complexes.

Autor:	Bergwell M; https://ror.org/035z6xf33 Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA., Park J; https://ror.org/035z6xf33 Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.; Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, OK, USA., Kirkland JG; https://ror.org/035z6xf33 Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA jake-kirkland@omrf.org.; Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, OK, USA.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2024 Aug 29; Vol. 7 (11). Date of Electronic Publication: 2024 Aug 29 (Print Publication: 2024).
DOI:	10.26508/lsa.202402715
Abstrakt:	Chromatin regulators alter the physical properties of chromatin to make it more or less permissive to transcription by modulating another protein's access to a specific DNA sequence through changes in nucleosome occupancy or histone modifications at a particular locus. Mammalian SWI/SNF complexes are a group of ATPase-dependent chromatin remodelers. In mouse embryonic stem cells, there are three primary forms of mSWI/SNF: canonical BAF (cBAF), polybromo-associated BAF (pBAF), and GLTSCR-associated BAF (gBAF). Nkx2-9 is bivalent, meaning nucleosomes at the locus have active and repressive modifications. In this study, we used unique BAF subunits to recruit each of the three complexes to Nkx2-9 using dCas9-mediated inducible recruitment (FIRE-Cas9). We show that recruitment of cBAF complexes leads to a significant loss of the polycomb repressive-2 H3K27me3 histone mark and polycomb repressive-1 and repressive-2 complex proteins, whereas gBAF and pBAF do not. Moreover, nucleosome occupancy alone cannot explain the loss of these marks. Our results demonstrate that cBAF has a unique role in the direct opposition of polycomb-associated histone modifications that gBAF and pBAF do not share. (© 2024 Bergwell et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu