"Investigation of the potential cellular changes induced by magnesium sulfate and salubrinal in a lipopolysaccharide-induced chorioamnionitis model".

Autor: Asci H; Suleyman Demirel University, Faculty of Medicine, Department of Pharmacology, Isparta, Turkey., Savran M; Suleyman Demirel University, Faculty of Medicine, Department of Pharmacology, Isparta, Turkey., Tepebasi MY; Suleyman Demirel University, Faculty of Medicine, Department of Medical Genetic, Isparta, Turkey., Ilhan I; Suleyman Demirel University, Faculty of Medicine, Department of Biochemistry, Isparta, Turkey., Karakuyu NF; Suleyman Demirel University, Faculty of Pharmacy, Department of Pharmacology, Isparta, Turkey., Imeci OB; Suleyman Demirel University, Faculty of Medicine, Department of Pharmacology, Isparta, Turkey. Electronic address: orhanimeci@sdu.edu.tr., Sevuk MA; Suleyman Demirel University, Faculty of Medicine, Department of Pharmacology, Isparta, Turkey., Sezik M; Suleyman Demirel University, Faculty of Medicine, Department of Obstetrics and Gynecology, Isparta, Turkey., Ozmen O; Burdur Mehmet Akif Ersoy University, Faculty of Veterinary, Department of Pathology, Burdur, Turkey.
Jazyk: angličtina
Zdroj: European journal of pharmacology [Eur J Pharmacol] 2024 Nov 05; Vol. 982, pp. 176949. Date of Electronic Publication: 2024 Aug 27.
DOI: 10.1016/j.ejphar.2024.176949
Abstrakt: Chorioamnionitis is closely associated with preterm labor and poses a significant public health concern. In this pathological process where inflammation plays a key role, intracellular mechanisms such as endoplasmic reticulum stress are crucial. In this study, we aimed to explore the potential positive outcomes of the combined use of salubrinal (SLB) with magnesium (Mg) treatment in chorioamnionitis. Thirty pregnant rats were divided into 5 groups as: Control, LPS (1 mg/kg), LPS + SLB (1 mg/kg), LPS + Mg (Dhaka protocol), LPS + SLB + Mg. Rats were sacrificed 4 h after LPS administration, then placental and fetal brain tissues were collected. LPS administration enhanced the levels of tumor necrosis factor-alpha, vascular endothelial growth factor, caspase-3 immunoexpressions, BAX, eukaryotic initiation factor 2-alpha, s100, and glial fibrillary acidic protein expressions and lowered BCL2 expressions in the placenta or fetal brains. SLB and Mg treatments were observed to reverse all these findings, and the most significant positive effect was in the LPS + SLB + Mg group. The known anti-inflammatory activity of Mg, when used with SLB, preventing the transition to apoptosis and increasing antioxidant enzyme activity, as identified in this study, can contribute significantly to the literature. However, these results need to be supported by additional molecular studies.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Halil Asci reports financial support was provided by Scientific and Technological Research Council of Turkey and Scientific Research Projects Coordination Unit of Suleyman Demirel University. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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