The Sec pathway gene yidC regulates the virulence of mesophilic Aeromonassalmonicida.

Autor: Yi X; State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China, Engineering Research Center of the Modern Technology for Eel Industry; Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, 524000, China., Xu X; State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China, Engineering Research Center of the Modern Technology for Eel Industry. Electronic address: Xiaojinxu@jmu.edu.cn., Xu G; State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China, Engineering Research Center of the Modern Technology for Eel Industry., Zhang Y; Institute of Electromagnetics and Acoustics, School of Electronic Science and Engineering, Xiamen University, Xiamen, Fujian, China. Electronic address: Zhangyouyu@xmu.edu.cn., Chen Y; State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China, Engineering Research Center of the Modern Technology for Eel Industry., Zhu Z; State Key Laboratory of Mariculture Breeding, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China, Engineering Research Center of the Modern Technology for Eel Industry., Guo M; South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Oct; Vol. 153, pp. 109863. Date of Electronic Publication: 2024 Aug 27.
DOI: 10.1016/j.fsi.2024.109863
Abstrakt: Aeromonas salmonicida is a common pathogenic bacterial species found in both freshwater and marine fish, leading to significant economic losses in the aquaculture industry. YidC is an accessory to SecYEG and is essential for the SecYEG transporter to insert into the bacterial membrane. However, the roles of the yidC gene on the host immune response remain unclear. Here, we compared the pathogenicity of yidC gene-deleted (ΔyidC) strain and wild-type (SRW-OG1) strain of mesophilic A. salmonicida to Orange-spotted grouper (Epinephelus coioides), and explored the impacts of yidC gene on the immune response of E. coioides to mesophilic A. salmonicida infection by using Red/ET recombineering. In this study, the E. coioides in the Secondary infected group had a 53.9 % higher survival rate than those in the Primary infected group. In addition, the adhesion ability of ΔyidC strain decreased by about 83.36 % compared with that of the wild-type (SRW-OG1) strain. Further comparison of the biological phenotype of SRW-OG1 and ΔyidC revealed that this yidC gene could regulate the expression of genes related to iron metabolism and have no effect on bacterial growth under the limited iron concentration. In the low concentration of Fe 3+ and Fe 2+ environment, SRW-OG1 can obtain iron ions by regulating yidC. Based on the above results, yidC gene contributed to the pathogenicity of mesophilic A. salmonicida to E. coioides, deletion of yidC gene promoted the inflammation and immune response of E. coioides to mesophilic A. salmonicida infection.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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