| Autor: |
Abe T; Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi 329-0498, Japan.; Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi 329-0498, Japan., Endo K; Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi 329-0498, Japan., Hanazono Y; Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi 329-0498, Japan.; Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi 329-0498, Japan., Kobayashi E; Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi 329-0498, Japan.; Department of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan. |
| Abstrakt: |
Luminescent technology based on the luciferin-luciferase reaction has been extensively employed across various disciplines as a quantitative imaging modality. Owing to its non-invasive imaging capacity, it has evolved as a valuable in vivo bioimaging tool, particularly in small animal models in fields such as gene and cell therapies. We have previously successfully generated rats with a systemic expression of the luciferase gene at the Rosa26 locus. In this study, we transplanted bone marrow from these rats into micro-mini pigs and used in vivo imaging to non-invasively analyze the dynamics of the transplanted cells. In addition, we established that the rat-to-pig transplantation system is a discordant system, similar to the pig-to-human transplantation system. Thus, rat-to-pig transplantation may provide a clinically appropriate large animal model for pig-to-human xenotransplantation. |
