| Autor: |
Grasso F; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy., Martínez MMA; ANFACO-CECOPESCA, Department of Circular Economy, Colexio Universitario, 36310 Vigo, Spain., Turrini F; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy.; National Center for the Development of New Technologies in Agriculture (Agritech), 80121 Napoli, Italy., Méndez Paz D; ANFACO-CECOPESCA, Department of Circular Economy, Colexio Universitario, 36310 Vigo, Spain., Vázquez Sobrado R; ANFACO-CECOPESCA, Department of Circular Economy, Colexio Universitario, 36310 Vigo, Spain., Orlandi V; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy., Jenssen M; Nofima, Muninbakken, 9-13, 9019 Tromsø, Norway., Lian K; Nofima, Muninbakken, 9-13, 9019 Tromsø, Norway., Rombi J; MICAMO LAB, Via XX Settembre 33/10, 16121 Genova, Italy., Tiso M; MICAMO LAB, Via XX Settembre 33/10, 16121 Genova, Italy., Razzuoli E; Sezione di Genova Portualità Marittima, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, 16129 Genova, Italy., Costas C; ANFACO-CECOPESCA, Department of Circular Economy, Colexio Universitario, 36310 Vigo, Spain., Boggia R; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy.; National Biodiversity Future Center (NBFC), 90133 Palermo, Italy. |
| Abstrakt: |
The aim of this research is to propose simple and scalable processes to obtain bioactive peptides extensively hydrolyzed starting from a tuna mixed biomass. The upcycling of this powdered biomass is challenging since it comes from the unsorted industrial side streams of the tuna canning process (cooked residues from fillet trimming) after a patented mild dehydration useful for preventing its degradation until its exploitation. Two different protocols were proposed, with and without the inclusion of an exogenous enzyme (Enzymatic-Assisted Extraction, EAE), with no relevant differences in yields (24% vs. 22%) and a comparable amino acid composition. Nevertheless, the former protocol (with EAE) provided peptides with an average molecular weight of 1.3 kDa, and the second one (without EAE) provided peptides with an average molecular weight of 2.2 kDa. The two corresponding types of tuna protein hydrolysates (Enzymatic Hydrolysates (EH) and Non-Enzymatic Hydrolysates (NEH)) were characterized by proximate compositions, pH, color profile, amino acid analysis, FTIR spectra, and molecular weight distribution. In addition, several biological analyses were performed to assess their potential use as nutraceutical supplements: special attention has been paid to antioxidant activity using three different methods to quantify it. EH showed the most promising antioxidant activity which could be exploited also in other fields (e.g., biomaterials, cosmetics). |
