Leaf Gas Film 1 promotes glycerol ester accumulation and formation of a tight root barrier to radial O2 loss in rice.

Autor:	Jiménez JC; Department of Biology, University of Copenhagen, Universitetsparken 4, 3rd floor, 2100 Copenhagen, Denmark.; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan., Noorrohmah S; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan.; Research Center for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Jl. Raya Bogor KM.46, Cibinong, Bogor, West Java 16911, Indonesia., Suresh K; Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany., Zeisler-Diehl VV; Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany., Peralta Ogorek LL; Department of Biology, University of Copenhagen, Universitetsparken 4, 3rd floor, 2100 Copenhagen, Denmark.; School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, the United Kingdom., Herzog M; Department of Biology, University of Copenhagen, Universitetsparken 4, 3rd floor, 2100 Copenhagen, Denmark., Pellegrini E; Department of Biology, University of Copenhagen, Universitetsparken 4, 3rd floor, 2100 Copenhagen, Denmark., Nagai K; Bioscience and Biotechnology Center of Nagoya University, Furo-Cho, Chikusa, Nagoya, Aichi 464-8602, Japan., Ashikari M; Bioscience and Biotechnology Center of Nagoya University, Furo-Cho, Chikusa, Nagoya, Aichi 464-8602, Japan., Takahashi H; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan., Pedersen O; Department of Biology, University of Copenhagen, Universitetsparken 4, 3rd floor, 2100 Copenhagen, Denmark.; School of Biological Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia., Schreiber L; Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany., Nakazono M; Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan.; School of Agriculture and Environment, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2024 Aug 28. Date of Electronic Publication: 2024 Aug 28.
DOI:	10.1093/plphys/kiae458
Abstrakt:	Rice (Oryza sativa L.) and many other wetland plants form an apoplastic barrier in the outer parts of the roots to restrict radial O2 loss to the rhizosphere during soil flooding. This barrier facilitates longitudinal internal O2 diffusion via gas-filled tissues from shoot to root apices, enabling root growth in anoxic soils. We tested the hypothesis that Leaf Gas Film 1 (LGF1), which influences leaf hydrophobicity in rice, plays a crucial role in tight outer apoplastic barriers formation in rice roots. We examined the roots of a rice mutant (dripping wet leaf 7, drp7) lacking functional LGF1, its wild type, and an LGF1 overexpression line for their capacity to develop outer apoplastic barriers that restrict radial O2 loss. We quantified the chemical composition of the outer part of the root and measured radial O2 diffusion from intact roots. The drp7 mutant exhibited a weak barrier to radial O2 loss compared to the wild type. However, introducing functional LGF1 into the mutant fully restored tight barrier function. The formation of a tight barrier to radial O2 loss was associated with increased glycerol ester levels in exodermal cells, rather than differences in total root suberization or lignification. These results demonstrate that, in addition to its role in leaf hydrophobicity regulation, LGF1 plays an important role in controlling the function of the outer apoplastic barriers in roots. Our study suggests that increased deposition of glycerol esters in the suberized root exodermis establishes a tight barrier to radial O2 loss in rice roots. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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