| Autor: |
Slusher AL; Department of Pediatrics, Yale School of Medicine, New Haven, CT 06519, USA., Visavadiya NP; Exercise Biochemistry Laboratory, Department of Exercise Science and Health Promotion, Florida Atlantic University, FACSM 777 Glades Road, FH11A-126B, Boca Raton, FL 33431, USA., Fico BG; Exercise Biochemistry Laboratory, Department of Exercise Science and Health Promotion, Florida Atlantic University, FACSM 777 Glades Road, FH11A-126B, Boca Raton, FL 33431, USA., Estébanez B; Institute of Biomedicine (BIOMED), University of León, 24071 León, Spain., Acevedo EO; Department of Kinesiology and Health Sciences, Virginia Commonwealth University, Richmond, VA 23220, USA., Huang CJ; Exercise Biochemistry Laboratory, Department of Exercise Science and Health Promotion, Florida Atlantic University, FACSM 777 Glades Road, FH11A-126B, Boca Raton, FL 33431, USA. |
| Abstrakt: |
The impact of cardiorespiratory fitness (VO 2max ) and obesity on indices of oxidative stress in plasma and circulating exosome-like extracellular vesicles (ELVs) were examined following acute exercise. Indices of oxidative stress in plasma and isolated plasma ELVs were examined in aerobically trained (NW-Tr; n = 15) and untrained (NW-UTr; n = 18) normal-weight individuals and aerobically untrained individuals with obesity (Ob-Utr; n = 10) prior to and immediately following acute maximal treadmill running. Following exercise, ELV flotillin-1 expression ( p = 0.008) and plasma total antioxidant capacity (TAC; p = 0.010) increased more in NW-UTr compared to NW-Tr and Ob-UTr participants, whereas plasma protein carbonyls (PC) decreased more in Ob-UTr compared to NW-Tr and NW-UTr groups. ELV glutathione (GSH) concentrations decreased more in NW-Tr compared to NW-UTr and Ob-UTr participants ( p = 0.009), whereas lipid peroxidase (LPO) concentrations increased more in Ob-UTr compared to NW-Tr and NW-UTr participants ( p = 0.003). Body mass index (BMI) was associated negatively with plasma TAC and PC ( p < 0.05) and positively with ELV LPO concentration responses ( p = 0.009). Finally, plasma-to-total (plasma + ELV) GSH ratios decreased in Ob-UTr compared to NW-Tr and NW-UTr participants ( p = 0.006), PC ratios increased in NW-Tr and NW-UTr compared to Ob-UTr subjects ( p = 0.008), and reactive oxygen/nitrogen species ratios increased in NW-UTr and decreased in Ob-UTr participants ( p < 0.001). BMI, independently of VO 2max , differentially regulates indices of oxidative stress within plasma and circulating ELVs prior to and immediately following acute maximal treadmill exercise. |
