S. mansoni -derived omega-1 prevents OVA-specific allergic airway inflammation via hampering of cDC2 migration.

Autor: Patente TA; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Gasan TA; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Scheenstra M; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Ozir-Fazalalikhan A; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Obieglo K; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Schetters S; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Verwaerde S; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Vergote K; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Otto F; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Wilbers RHP; Laboratory of Nematology, Plant Sciences Group, Wageningen University and Research, Wageningen, Netherlands., van Bloois E; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Wijck YV; Department of Pulmonology, LUMC, Leiden, Netherlands., Taube C; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Hammad H; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.; Department of Internal Medicine, Ghent University, Ghent, Belgium., Schots A; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium., Everts B; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Yazdanbakhsh M; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Guigas B; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Hokke CH; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands., Smits HH; Department of Parasitology, Leiden University Center of Infectious Disease (LU-CID), Leiden University Medical Center (LUMC), Leiden, Netherlands.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2024 Aug 26; Vol. 20 (8), pp. e1012457. Date of Electronic Publication: 2024 Aug 26 (Print Publication: 2024).
DOI: 10.1371/journal.ppat.1012457
Abstrakt: Chronic infection with Schistosoma mansoni parasites is associated with reduced allergic sensitization in humans, while schistosome eggs protects against allergic airway inflammation (AAI) in mice. One of the main secretory/excretory molecules from schistosome eggs is the glycosylated T2-RNAse Omega-1 (ω1). We hypothesized that ω1 induces protection against AAI during infection. Peritoneal administration of ω1 prior to sensitization with Ovalbumin (OVA) reduced airway eosinophilia and pathology, and OVA-specific Th2 responses upon challenge, independent from changes in regulatory T cells. ω1 was taken up by monocyte-derived dendritic cells, mannose receptor (CD206)-positive conventional type 2 dendritic cells (CD206+ cDC2), and by recruited peritoneal macrophages. Additionally, ω1 impaired CCR7, F-actin, and costimulatory molecule expression on myeloid cells and cDC2 migration in and ex vivo, as evidenced by reduced OVA+ CD206+ cDC2 in the draining mediastinal lymph nodes (medLn) and retainment in the peritoneal cavity, while antigen processing and presentation in cDC2 were not affected by ω1 treatment. Importantly, RNAse mutant ω1 was unable to reduce AAI or affect DC migration, indicating that ω1 effects are dependent on its RNAse activity. Altogether, ω1 hampers migration of OVA+ cDC2 to the draining medLn in mice, elucidating how ω1 prevents allergic airway inflammation in the OVA/alum mouse model.
Competing Interests: HS receive research grants from the Lung foundation Netherlands and the Dutch Research Council and is a board member of the Netherlands Respiratory Society.
(Copyright: © 2024 Patente et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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