Structural and functional bacterial biodiversity in a copper, zinc and nickel amended bioreactor:  shotgun metagenomic study.

Autor: Velázquez-Fernández JB; Department of Biotechnology and Bioengineering, Center for Research and Avanced Studies of the National Polythecnic Institute, Cinvestav Mexico City, Mexico. jesusb.velazquez@cinvestav.mx.; Investigador Por México, CONAHCYT, Mexico City, Mexico. jesusb.velazquez@cinvestav.mx., Aceves Suriano CE; Department of Biotechnology and Bioengineering, Center for Research and Avanced Studies of the National Polythecnic Institute, Cinvestav Mexico City, Mexico., Thalasso F; Department of Biotechnology and Bioengineering, Center for Research and Avanced Studies of the National Polythecnic Institute, Cinvestav Mexico City, Mexico., Montoya-Ciriaco N; Department of Biotechnology and Bioengineering, Center for Research and Avanced Studies of the National Polythecnic Institute, Cinvestav Mexico City, Mexico.; Doctorado en Ciencias Biológicas Centro Tlaxcala de Biología de La Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, Mexico., Dendooven L; Department of Biotechnology and Bioengineering, Center for Research and Avanced Studies of the National Polythecnic Institute, Cinvestav Mexico City, Mexico.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2024 Aug 24; Vol. 24 (1), pp. 313. Date of Electronic Publication: 2024 Aug 24.
DOI: 10.1186/s12866-024-03437-8
Abstrakt: Background: At lower concentrations copper (Cu), zinc (Zn) and nickel (Ni) are trace metals essential for some bacterial enzymes. At higher concentrations they might alter and inhibit microbial functioning in a bioreactor treating wastewater. We investigated the effect of incremental concentrations of Cu, Zn and Ni on the bacterial community structure and their metabolic functions by shotgun metagenomics. Metal concentrations reported in previous studies to inhibit bacterial metabolism were investigated.
Results: At 31.5 μM Cu, 112.4 μM Ni and 122.3 μM Zn, the most abundant bacteria were Achromobacter and Agrobacterium. When the metal concentration increased 2 or fivefold their abundance decreased and members of Delftia, Stenotrophomonas and Sphingomonas dominated. Although the heterotrophic metabolic functions based on the gene profile was not affected when the metal concentration increased, changes in the sulfur biogeochemical cycle were detected. Despite the large variations in the bacterial community structure when concentrations of Cu, Zn and Ni increased in the bioreactor, functional changes in carbon metabolism were small.
Conclusions: Community richness and diversity replacement indexes decreased significantly with increased metal concentration. Delftia antagonized Pseudomonas and members of Xanthomonadaceae. The relative abundance of most bacterial genes remained unchanged despite a five-fold increase in the metal concentration, but that of some EPS genes required for exopolysaccharide synthesis, and those related to the reduction of nitrite to nitrous oxide decreased which may alter the bioreactor functioning.
(© 2024. The Author(s).)
Databáze: MEDLINE
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