Propagating pluripotency - The conundrum of self-renewal.

Autor: Smith A; Living Systems Institute, University of Exeter, Exeter, UK.
Jazyk: angličtina
Zdroj: BioEssays : news and reviews in molecular, cellular and developmental biology [Bioessays] 2024 Dec; Vol. 46 (12), pp. e2400108. Date of Electronic Publication: 2024 Aug 23.
DOI: 10.1002/bies.202400108
Abstrakt: The discovery of mouse embryonic stem cells in 1981 transformed research in mammalian developmental biology and functional genomics. The subsequent generation of human pluripotent stem cells (PSCs) and the development of molecular reprogramming have opened unheralded avenues for drug discovery and cell replacement therapy. Here, I review the history of PSCs from the perspective that long-term self-renewal is a product of the in vitro signaling environment, rather than an intrinsic feature of embryos. I discuss the relationship between pluripotent states captured in vitro to stages of epiblast in the embryo and suggest key considerations for evaluation of PSCs. A remaining fundamental challenge is to determine whether naïve pluripotency can be propagated from the broad range of mammals by exploiting common principles in gene regulatory architecture.
(© 2024 The Author(s). BioEssays published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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