In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity.

Autor: Rooda I; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden. ilmatar.rooda@ki.se.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden. ilmatar.rooda@ki.se.; Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia. ilmatar.rooda@ki.se., Hassan J; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden., Hao J; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden.; Department of Reproductive Medicine, Xiangya Hospital, Central South University, Changsha, PR China., Wagner M; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden., Moussaud-Lamodière E; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden., Jääger K; Institute of Computer Science, University of Tartu, Tartu, Estonia.; Competence Centre on Health Technologies, Tartu, Estonia., Otala M; Department of Obstetrics and Gynecology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Knuus K; Department of Obstetrics and Gynecology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Lindskog C; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Papaikonomou K; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden.; Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Gidlöf S; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden.; Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Langenskiöld C; Department of Pediatric Oncology, Queen Silvia Children's Hospital, Sahlgrenska University Hospital, Gothenburg, Sweden., Vogt H; Crown Princess Victoria Children's Hospital, and Division of Children's and Women's Health, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden., Frisk P; Department of Women's and Children's Health, Uppsala University Children's Hospital, Uppsala, Sweden., Malmros J; Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden., Tuuri T; Department of Obstetrics and Gynecology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Salumets A; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden.; Competence Centre on Health Technologies, Tartu, Estonia.; Department of Obstetrics and Gynaecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia., Jahnukainen K; Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.; Department of Women's and Children's Health, NORDFERTIL Research Lab Stockholm, Karolinska Institutet and University Hospital, Stockholm, Sweden., Velthut-Meikas A; Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia., Damdimopoulou P; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden. pauliina.damdimopoulou@ki.se.; Department of Gynecology and Reproductive Medicine, Karolinska University Hospital, Stockholm, Sweden. pauliina.damdimopoulou@ki.se.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Aug 21; Vol. 15 (1), pp. 6989. Date of Electronic Publication: 2024 Aug 21.
DOI: 10.1038/s41467-024-51185-0
Abstrakt: The ovarian cortical reserve of follicles is vital for fertility. Some medical treatments are toxic to follicles, leading to premature ovarian insufficiency. Ovarian tissue cryopreservation is an established method to preserve fertility in adults and even applied in prepuberty despite unproven efficacy. Here, we analyze transcriptomes of 120 cortical follicles from children and adults for detailed comparison. We discover heterogeneity with two main types of follicles in both age groups: one with expected oocyte-granulosa profiles and another with predicted role in signaling. Transcriptional changes during growth to the secondary stage are similar overall in children and adults, but variations related to extracellular matrix, theca cells, and miRNA profiles are found. Notably, cyclophosphamide dose correlates with interferon signaling in child follicles. Additionally, morphology alone is insufficient for follicle categorization suggesting a need for additional markers. Marker genes for early follicle activation are determined. These findings will help refine follicular classification and fertility preservation techniques across critical ages.
(© 2024. The Author(s).)
Databáze: MEDLINE
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