Development of alginate/chitosan hydrogel loaded with obestatin and evaluation of collagen type I, III, VEGF and TGF-β 1 gene expression for skin repair in a rat model (in vitro and in vitro study).

Autor: Toumaj N; Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran., Salehi M; Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.; Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran., Zamani S; Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran., Arabpour Z; Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, USA., Djalian AR; Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, USA., Rahmati M; Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Jazyk: angličtina
Zdroj: Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) [Skin Res Technol] 2024 Aug; Vol. 30 (8), pp. e70018.
DOI: 10.1111/srt.70018
Abstrakt: Background: Skin injuries have long been recognized as a prevalent type of physical injury. As a result, numerous research studies have been performed to discover an effective mechanism for wound healing. Therefore, tissue engineering of skin has developed as a potential solution for traditional methods of treating skin injuries.
Methods and Materials: Alginate/Chitosan hydrogel was mixed with 1, 10, 100, and 150 µM Obestatin, and evaluated the morphology, cumulative release, hemocompatibility and cytocompatibility, water absorption, cell viability, weight loss, and antibacterial characteristics of three-dimensional (3D) alginate (Alg) and chitosan (Cs) hydrogels during the process of wound curing. Various concentrations of Obestatin (Obes) were utilized for this purpose. Finally, the hydrogels that were made were tested on a full-thickness dermal wound in a Wistar rat model. The curative effects were determined by analyzing RNA expression and examining tissue stained with Masson's trichrome (MT) and hematoxylin-eosin (H&E).
Results: The biodegradability of this hydrogel was verified using weight loss testing, which demonstrated a reduction of around 90% after a period of 3 days. Furthermore, the MTT assay demonstrated that hydrogels have a beneficial effect on cell proliferation without inducing any harmful effects. Furthermore, the hydrogels produced demonstrated higher wound closure in vivo compared to the wounds treated with gauze (negative control group). Among the hydrogel groups, the chitosan/alginate/obestatin 100 µM group exhibited the apical percentage of wound closure, gene expression, and secondary epithelialization, but in 150 µM concentrations, we saw a lower rate of cell growth and proliferation and increase in hemolysis. In addition, RT-PCR analysis demonstrated that a concentration of 100 µM obestatin resulted in an upregulation in the expression of mRNA for vascular endothelial growth factor (VEGF), collagen type I & type III, and transforming growth factor-beta (TGF-β).
Conclusion: The present study suggests that 3D Alg/Cs hydrogels with a concentration of 100 µM obestatin have the potential for clinical application in the treatment of skin injuries.
(© 2024 The Author(s). Skin Research and Technology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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