Genetic evidence for a regulated cysteine protease catalytic triad in LegA7, a Legionella pneumophila protein that impinges on a stress response pathway.

Autor:	Hershkovitz D; The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel., Chen EJ; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.; Program in Genetics, Molecular and Cellular Biology, Graduate School of Biomedical Sciences Tufts University School of Medicine, Boston, Massachusetts, USA., Ensminger AW; Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada.; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada., Dugan AS; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA., Conway KT; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.; Program in Genetics, Molecular and Cellular Biology, Graduate School of Biomedical Sciences Tufts University School of Medicine, Boston, Massachusetts, USA., Joyce AC; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA., Segal G; The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel., Isberg RR; Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
Jazyk:	angličtina
Zdroj:	MSphere [mSphere] 2024 Sep 25; Vol. 9 (9), pp. e0022224. Date of Electronic Publication: 2024 Aug 21.
DOI:	10.1128/msphere.00222-24
Abstrakt:	Legionella pneumophila grows within membrane-bound vacuoles in phylogenetically diverse hosts. Intracellular growth requires the function of the Icm/Dot type-IVb secretion system, which translocates more than 300 proteins into host cells. A screen was performed to identify L. pneumophila proteins that stimulate mitogen-activated protein kinase (MAPK) activation, using Icm/Dot translocated proteins ectopically expressed in mammalian cells. In parallel, a second screen was performed to identify L. pneumophila proteins expressed in yeast that cause growth inhibition in MAPK pathway-stimulatory high-osmolarity medium. LegA7 was shared in both screens, a protein predicted to be a member of the bacterial cysteine protease family that has five carboxyl-terminal ankyrin repeats. Three conserved residues in the predicted catalytic triad of LegA7 were mutated. These mutations abolished the ability of LegA7 to inhibit yeast growth. To identify other residues important for LegA7 function, a generalizable selection strategy in yeast was devised to isolate mutants that have lost function and no longer cause growth inhibition on a high-osmolarity medium. Mutations were isolated in the two carboxyl-terminal ankyrin repeats, as well as an inter-domain region located between the cysteine protease domain and the ankyrin repeats. These mutations were predicted by AlphaFold modeling to localize to the face opposite from the catalytic site, arguing that they interfere with the positive regulation of the catalytic activity. Based on our data, we present a model in which LegA7 harbors a cysteine protease domain with an inter-domain and two carboxyl-terminal ankyrin repeat regions that modulate the function of the catalytic domain. Importance: Legionella pneumophila grows in a membrane-bound compartment in macrophages during disease. Construction of the compartment requires a dedicated secretion system that translocates virulence proteins into host cells. One of these proteins, LegA7, is shown to activate a stress response pathway in host cells called the mitogen-activated protein kinase (MAPK) pathway. The effects on the mammalian MAPK pathway were reconstructed in yeast, allowing the development of a strategy to identify the role of individual domains of LegA7. A domain similar to cysteine proteases is demonstrated to be critical for impinging on the MAPK pathway, and the catalytic activity of this domain is required for targeting this path. In addition, a conserved series of repeats, called ankyrin repeats, controls this activity. Data are provided that argue the interaction of the ankyrin repeats with unknown targets probably results in activation of the cysteine protease domain. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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