Evaluating the embryotoxicity of benzophenone-based photoinitiators in stem cells and zebrafish embryos.

Autor: Weng CY; National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Taiwan., Chang TC; Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, Taiwan., Liou JY; Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan, Taiwan., Hsu JH; National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Taiwan., Ho CC; National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Taiwan., Arrokhman S; National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Taiwan., Lin P; National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan, Taiwan. Electronic address: pplin@nhri.org.tw.
Jazyk: angličtina
Zdroj: Toxicology [Toxicology] 2024 Nov; Vol. 508, pp. 153930. Date of Electronic Publication: 2024 Aug 17.
DOI: 10.1016/j.tox.2024.153930
Abstrakt: Benzophenones (BPs) are widely used as photoinitiators (PIs) or printing inks in food packaging, which may migrate into foods. However, the toxicity information of some BP analogues, such as 4,4'-bis(diethylamino)-benzophenone (DEAB), 4-phenylbenzophenone (4-PBP), 4 (hydroxymethyl)benzophenone (4-HMBP), those are used as PIs is lacking. Developmental toxicity is a health concern associated with PIs exposure. Recently, alternative non-in vivo methods have been proposed to evaluate the concerned chemicals or better understand the modes of action of certain toxicological endpoints. In this study, using in silico methods, we predicted that BP, DEAB, 4-PBP and 4-HMBP might exhibit developmental toxicity. However, we found that only DEAB is strong embryotoxic and disturbs the early differentiation of mouse embryonic stem cells into three germ layers and cardiomyocytes. DEAB treatment also prevented cardiomyocyte differentiation in human induced pluripotent stem cells (hiPSCs) on day 10. However, BP, 4-PBP and 4-HMBP had no similar effects on cardiomyocyte differentiation on day 10. Transcriptomic analysis revealed that treatment with DEAB significantly decreased the mRNA levels of differentiation-related transcription factors SOX17 and FOXA1, in hiPSCs on day 4. Furthermore, DEAB treatment caused tail malformations and yolk sac edema in zebrafish embryos. To conclude, DEAB may be embryotoxic because it disturbs the early differentiation of stem cells. Further studies are warranted to better understand the health effects of DEAB exposure.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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