| Autor: |
Debruin D; Department of Medicine - Western Health, The University of Melbourne, Melbourne, Victoria, Australia.; Institute for Health and Sport (IHeS), Victoria University, Melbourne, Victoria, Australia., McRae NL; Centre for Molecular and Medical Research, School of Medicine, Deakin University, Geelong, Victoria, Australia., Addinsall AB; Centre for Molecular and Medical Research, School of Medicine, Deakin University, Geelong, Victoria, Australia.; Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark., McCulloch DR; Centre for Molecular and Medical Research, School of Medicine, Deakin University, Geelong, Victoria, Australia., Barker RG; Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, Victoria, Australia., Debrincat D; Institute for Health and Sport (IHeS), Victoria University, Melbourne, Victoria, Australia., Hayes A; Department of Medicine - Western Health, The University of Melbourne, Melbourne, Victoria, Australia.; Institute for Health and Sport (IHeS), Victoria University, Melbourne, Victoria, Australia.; Australian Institute for Musculoskeletal Sciences (AIMSS), Victoria University & Western Health, Melbourne, Victoria, Australia., Murphy RM; Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, Victoria, Australia., Stupka N; Department of Medicine - Western Health, The University of Melbourne, Melbourne, Victoria, Australia.; Institute for Health and Sport (IHeS), Victoria University, Melbourne, Victoria, Australia. |
| Abstrakt: |
Versican is increased with inflammation and fibrosis, and is upregulated in Duchenne muscular dystrophy. In fibrotic diaphragm muscles from dystrophic mdx mice, genetic reduction of versican attenuated macrophage infiltration and improved contractile function. Versican is also implicated in myogenesis. Here, we investigated whether versican modulated mdx hindlimb muscle pathology, where inflammation and regeneration are increased but fibrosis is minimal. Immunohistochemistry and qRT-PCR were used to assess how fiber type and glucocorticoids (α-methylprednisolone) modify versican expression. To genetically reduce versican, female mdx and male versican haploinsufficient (hdf) mice were bred resulting in male mdx -hdf and mdx (control) pups. Versican expression, contractile function, and pathology were evaluated in hindlimb muscles. Versican immunoreactivity was greater in slow versus fast hindlimb muscles. Versican mRNA transcripts were reduced by α-methylprednisolone in soleus, but not in fast extensor digitorum longus, muscles. In juvenile (6-wk-old) mdx -hdf mice, versican expression was most robustly decreased in soleus muscles leading to improved force output and a modest reduction in fatiguability. These functional benefits were not accompanied by decreased inflammation. Muscle architecture, regeneration markers, and fiber type also did not differ between mdx -hdf mice and mdx littermates. Improvements in soleus contractile function were not retained in adult (20-wk-old) mdx -hdf mice. In conclusion, soleus muscles from juvenile mdx mice were most responsive to pharmacological or genetic approaches targeting versican; however, the benefits of versican reduction were limited due to low fibrosis. Preclinical matrix research in dystrophy should account for muscle phenotype (including age) and the interdependence between inflammation and fibrosis. NEW & NOTEWORTHY The proteoglycan versican is upregulated in muscular dystrophy. In fibrotic diaphragm muscles from mdx mice, versican reduction attenuated macrophage infiltration and improved performance. Here, in hindlimb muscles from 6- and 20-wk-old mdx mice, where pathology is mild, versican reduction did not decrease inflammation and contractile function improvements were limited to juvenile mice. In dystrophic mdx muscles, the association between versican and inflammation is mediated by fibrosis, demonstrating interdependence between the immune system and extracellular matrix. |
