Development of a new extraction method and functional analysis of phycocyanobilin from unique filamentous cyanobacteria.

Autor: Aoki J; College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan., Yarita T; College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan., Hasegawa M; College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan., Asayama M; College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan. Electronic address: munehiko.asayama.777@vc.ibaraki.ac.jp.
Jazyk: angličtina
Zdroj: Journal of biotechnology [J Biotechnol] 2024 Nov 20; Vol. 395, pp. 180-188. Date of Electronic Publication: 2024 Aug 15.
DOI: 10.1016/j.jbiotec.2024.08.006
Abstrakt: As current methods of production of phycocyanobilin, a photosynthetic blue pigment derived from phycocyanin of filamentous cyanobacteria, Pseudanabaena sp. ABRG5-3, Limnothrix sp. SK1-2-1, and Spirulina sp., exhibit a low extraction efficiency, a new extraction method using ethanol extraction as a type of solvolysis with an autoclave (130 ℃, 5.7 bar, 10 min) was developed in this study. This method exhibited high efficiency and enabled easy recovery of the three types of phycocyanobilins. The identity of the three types of phycocyanobilins was confirmed by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry. Phycocyanobilins were stable at high temperatures (80 ℃) and acidic (pH 3) conditions. Phycocyanobilins also possessed a remarkable antioxidant property. This is the first time that a simple phycocyanobilin extraction method with a recovery rate of more than 60 % and approximately 1 % per dry cell weight of filamentous cyanobacteria has been demonstrated. This novel production method is thus convenient and effective for obtaining high-purity phycocyanobilins.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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