Inside Mycobacterium bovis SB0120 spoligotype circulating in Italy: analysis of the most frequent genotypes by whole genome sequencing.

Autor:	Scaltriti E; Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale Della Lombardia e Dell'Emilia-Romagna (IZSLER), Parma, Italy., Iyad K; National Reference Centre for Bovine Tuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - IZSLER, Brescia, Italy., Boniotti MB; National Reference Centre for Bovine Tuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - IZSLER, Brescia, Italy., Menozzi I; Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale Della Lombardia e Dell'Emilia-Romagna (IZSLER), Parma, Italy., Bolzoni L; Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale Della Lombardia e Dell'Emilia-Romagna (IZSLER), Parma, Italy., Ippolito D; Area Territoriale Barcellona Pozzo di Gotto, Istituto Zooprofilattico Sperimentale della Sicilia 'A. Mirri', Barcellona Pozzo di Gotto, Messina, Italy., Ciarello FP; Area Territoriale Barcellona Pozzo di Gotto, Istituto Zooprofilattico Sperimentale della Sicilia 'A. Mirri', Barcellona Pozzo di Gotto, Messina, Italy., Loda D; National Reference Centre for Bovine Tuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - IZSLER, Brescia, Italy., D'Incau M; National Reference Centre for Bovine Tuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - IZSLER, Brescia, Italy., Zanoni M; National Reference Centre for Bovine Tuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - IZSLER, Brescia, Italy., Presti VDML; Area Territoriale Barcellona Pozzo di Gotto, Istituto Zooprofilattico Sperimentale della Sicilia 'A. Mirri', Barcellona Pozzo di Gotto, Messina, Italy., Mazzone P; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati' (IZSUM), Perugia, Italy., Gavaudan S; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati' (IZSUM), Ancona, Italy., Pacciarini ML; National Reference Centre for Bovine Tuberculosis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - IZSLER, Brescia, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Jul 26; Vol. 15, pp. 1416605. Date of Electronic Publication: 2024 Jul 26 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1416605
Abstrakt:	Bovine tuberculosis (bTB) is a chronic inflammatory disease primarily caused by Mycobacterium bovis . The infection affects domestic animals and wildlife, posing a zoonotic risk to humans. To understand the dynamics of transmission and genetic diversity in Italy's M. bovis population, we conducted whole-genome sequencing (WGS) analysis on two prevalent genotypes, belonging to Spoligotype SB0120, identified in different geographical and temporal contexts. By comparing these genomes with international M. bovis isolates, we identified a distinct clade within the lineage La1.2, encompassing the Italian SB0120 isolates, indicating a genomic segregation of Italian M. bovis from other European isolates. Within Italy, a significant level of genetic variability emerged across regions, while isolates within epidemiologically linked outbreaks exhibited minimal genetic diversity. Additionally, isolates derived from cattle and wild boars within a tuberculosis hotspot in Central Italy and from cattle and black pigs in Sicily formed unified clonal clusters. This indicates the presence of persistent strains circulating in the examined regions. The genetic diversity within herds was limited, as specific clones endured over time within certain herds. This research enhances our comprehension of the epidemiology and transmission patterns of bTB in Italy, thereby aiding the development of precise control strategies and disease management. Using WGS and implementing standardized protocols and databases will be pivotal in combating bTB and promoting One-Health approaches to address this noteworthy public health concern. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Scaltriti, Iyad, Boniotti, Menozzi, Bolzoni, Ippolito, Ciarello, Loda, D’Incau, Zanoni, Presti, Mazzone, Gavaudan and Pacciarini.)
Databáze:	MEDLINE
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