Astragaloside IV attenuates ferroptosis and protects against iron overload-induced retinal injury.

Autor: Li Q; Eye School of Chengdu University of Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China; Ineye Hospital of Chengdu University of Traditional Chinese Medicine, No.8 Xinghui Road, Chengdu, 610084, Sichuan, China; Key Laboratory of Sichuan Province Ophthalmopathy Prevention & Cure and Visual Function Protection with Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China; Guangzhou Ineye Vision Health Innovation Institute, No.2 Fenghuang 3rd Road, Guangzhou, 510555, Guangdong, China., Zhang Y; Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, No.326 Xinshi South Road, Shijiazhuang, 050200, Hebei, China., Liu P; Department of General Surgery, Chengdu First People's Hospital/Chengdu Integrated TCM & Western Medicine Hospital, No. 18, Wangxiang North Road, Chengdu, 610041, Sichuan, China., Wang C; Eye School of Chengdu University of Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China., Pan Y; Sichuan Vocational College of Health and Rehabilitation, No.3 Deming Road, Zigong, 643030, Sichuan, China., Nie Y; Eye School of Chengdu University of Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China; Ineye Hospital of Chengdu University of Traditional Chinese Medicine, No.8 Xinghui Road, Chengdu, 610084, Sichuan, China., Tang W; Eye School of Chengdu University of Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China., Wang Q; Eye School of Chengdu University of Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China; Ineye Hospital of Chengdu University of Traditional Chinese Medicine, No.8 Xinghui Road, Chengdu, 610084, Sichuan, China; Key Laboratory of Sichuan Province Ophthalmopathy Prevention & Cure and Visual Function Protection with Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China., Song Q; Eye School of Chengdu University of Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China; Ineye Hospital of Chengdu University of Traditional Chinese Medicine, No.8 Xinghui Road, Chengdu, 610084, Sichuan, China; Key Laboratory of Sichuan Province Ophthalmopathy Prevention & Cure and Visual Function Protection with Traditional Chinese Medicine, No.37 Twelve Bridge Road, Chengdu, 610075, Sichuan, China; Guangzhou Ineye Vision Health Innovation Institute, No.2 Fenghuang 3rd Road, Guangzhou, 510555, Guangdong, China. Electronic address: songqt607@cdutcm.edu.cn.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2024 Sep; Vol. 246, pp. 110021. Date of Electronic Publication: 2024 Aug 06.
DOI: 10.1016/j.exer.2024.110021
Abstrakt: Retinal injury may be exacerbated by iron overload. Astragaloside IV (AS-IV) has potential applications in the food and healthcare industry to promote eye health. We sought to determine the mechanisms responsible for the protective effects of AS-IV on photoreceptor and retinal pigment epithelium cell death induced by iron overload. We conducted in vitro and in vivo experiments involving AS-IV pretreatment. We tested AS-IV for its ability to protect iron-overload mice from retinal injury. In particular, we analyzed the effects of AS-IV on iron overload-induced ferroptosis in 661W and ARPE-19 cells. AS-IV not only attenuated iron deposition and retinal injury in iron-overload mice but also effectively reduced iron overload-induced ferroptotic cell death in 661W and ARPE-19 cells. AS-IV effectively prevented ferroptosis by inhibiting iron accumulation and lipid peroxidation. In addition, inhibiting nuclear factor erythroid 2-related factor 2 (Nrf2) eliminated the protective effect of AS-IV against ferroptosis. The results suggest that ferroptosis might be a significant cause of retinal cell death associated with iron overload. AS-IV provides protection from iron overload-induced ferroptosis, partly by activating the Nrf2 signaling pathway.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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