Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress.

Autor:	Chen M; Department of Ecology, Earth & Environmental Sciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Trotter VV; Division of Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Walian PJ; Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Chen Y; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Lopez R; Department of Ecology, Earth & Environmental Sciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Lui LM; Division of Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Nielsen TN; Division of Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Malana RG; Department of Ecology, Earth & Environmental Sciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Thorgersen MP; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA., Hendrickson AJ; Division of Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Carion H; Division of Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Deutschbauer AM; Division of Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, 94720, USA., Petzold CJ; Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Smith HJ; Center for Biofilm Engineering and Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, 59717, USA., Arkin AP; Division of Environmental Genomics and Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA., Adams MWW; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, 30602, USA., Fields MW; Center for Biofilm Engineering and Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, 59717, USA., Chakraborty R; Department of Ecology, Earth & Environmental Sciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Jazyk:	angličtina
Zdroj:	The ISME journal [ISME J] 2024 Jan 08; Vol. 18 (1).
DOI:	10.1093/ismejo/wrae151
Abstrakt:	Biofilms aid bacterial adhesion to surfaces via direct and indirect mechanisms, and formation of biofilms is considered as an important strategy for adaptation and survival in suboptimal environmental conditions. However, the molecular underpinnings of biofilm formation in subsurface sediment/groundwater ecosystems where microorganisms often experience fluctuations in nutrient input, pH, and nitrate or metal concentrations are underexplored. We examined biofilm formation under different nutrient, pH, metal, and nitrate regimens of 16 Rhodanobacter strains isolated from subsurface groundwater wells spanning diverse levels of pH (3.5 to 5) and nitrates (13.7 to 146 mM). Eight Rhodanobacter strains demonstrated significant biofilm growth under low pH, suggesting adaptations for survival and growth at low pH. Biofilms were intensified under aluminum stress, particularly in strains possessing fewer genetic traits associated with biofilm formation, findings warranting further investigation. Through random barcode transposon-site sequencing (RB-TnSeq), proteomics, use of specific mutants, and transmission electron microscopy analysis, we discovered flagellar loss under aluminum stress, indicating a potential relationship between motility, metal tolerance, and biofilm growth. Comparative genomic analyses revealed the absence of flagella and chemotaxis genes and the presence of a putative type VI secretion system in the highly biofilm-forming strain FW021-MT20. In this study we identified genetic determinants associated with biofilm growth under metal stress in a predominant environmental genus, Rhodanobacter, and identified traits aiding survival and adaptation to contaminated subsurface environments. (Published by Oxford University Press on behalf of the International Society for Microbial Ecology 2024.)
Databáze:	MEDLINE
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