Measurement of potassium in rats using an In vivo neutron activation analysis system.

Autor: Tabbassum S; School of Health Sciences, Purdue University, West Lafayette, IN, USA. Electronic address: stabbass@purdue.edu., Yue S; School of Health Sciences, Purdue University, West Lafayette, IN, USA., Cheng P; School of Health Sciences, Purdue University, West Lafayette, IN, USA; School of Nuclear Science and Technology, Nanhua University, Henyang, Hunan, China., Yanko F; School of Health Sciences, Purdue University, West Lafayette, IN, USA; Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Balachandran R; School of Health Sciences, Purdue University, West Lafayette, IN, USA; Exponent Inc, Alexandria, VA, USA., Aschner M; Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA., Bowman AB; School of Health Sciences, Purdue University, West Lafayette, IN, USA., Nie LH; School of Health Sciences, Purdue University, West Lafayette, IN, USA. Electronic address: hine@purdue.edu.
Jazyk: angličtina
Zdroj: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine [Appl Radiat Isot] 2024 Oct; Vol. 212, pp. 111439. Date of Electronic Publication: 2024 Jul 18.
DOI: 10.1016/j.apradiso.2024.111439
Abstrakt: Abnormal levels of potassium are linked to several health conditions, including high blood pressure, cardiac dysfunction, kidney damage, and osteoporosis. Given the limited availability of in vivo measurement techniques, there is a need for novel methods to measure potassium to enhance the diagnosis and management of potassium metabolism related diseases. This study aimed to evaluate the feasibility of compact neutron generator based in vivo measurement system for quantification of potassium using rat carcasses. A cohort of thirty-nine rats (n = 20 males and 19 females, average weight 255 ± 15 and 163 ± 7 g) were sacrificed, and their carcasses were placed in polyethylene bottles. The rats were then positioned and irradiated in a carefully designed irradiation cave built alongside the neutron generator with an optimized thermal neutron flux and radiation dose ratio. The irradiation time was 10 min, followed by a 5-min decay and 2-h measurement using a high efficiency high purity germanium detector(HPGe). RESULTS: The average potassium concentration in male and female rats was found to be comparable (male 2874 ± 161 and female 2866 ± 144 μg/g). A marginally positive correlation between potassium concentration and weight was found in female rats only (male(20) = 0.07, P = 0.76 and female r(19) = 0.34, P = 0.15). We assessed the influence of manganese toxicity on potassium levels and observed no significant impact. These results were consistent with our previous study in mice. CONCLUSION: This study suggests that in vivo neutron activation analysis could serve as a promising method to quantify potassium and to investigate the storage and metabolism of potassium in human and in animals.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Michael Aschner and Aaron B. Bowman reports financial support was provided by National Institute of Environmental Health Sciences.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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