Pseudomonas syringae pv. actinidiae Unique Effector HopZ5 Interacts with GF14C to Trigger Plant Immunity.

Autor:	Zhou M; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi, China.; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China., Zhang J; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi, China.; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China., Zhao Z; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi, China.; Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China., Liu W; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi, China.; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China., Wu Z; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi, China.; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China., Huang L; State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling 712100, Shaanxi, China.; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
Jazyk:	angličtina
Zdroj:	Phytopathology [Phytopathology] 2024 Oct; Vol. 114 (10), pp. 2322-2330. Date of Electronic Publication: 2024 Oct 04.
DOI:	10.1094/PHYTO-09-23-0330-R
Abstrakt:	The bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae ( Psa ) is the most devastating disease threatening the global kiwifruit production. This pathogen delivers multiple effector proteins into plant cells to resist plant immune responses and facilitate their survival. Here, we focused on the unique effector HopZ5 in Psa , which previously has been reported to have virulence functions. In this study, our results showed that HopZ5 could cause macroscopic cell death and trigger a serious immune response by agroinfiltration in Nicotiana benthamiana , along with upregulated expression of immunity-related genes and significant accumulation of reactive oxygen species and callose. Subsequently, we confirmed that HopZ5 interacted with the phosphoserine-binding protein GF14C in both the nonhost plant N. benthamiana (NbGF14C) and the host plant kiwifruit (AcGF14C), and silencing of NbGF14C compromised HopZ5-mediated cell death, suggesting that GF14C plays a crucial role in the detection of HopZ5. Further studies showed that overexpression of NbGF14C both markedly reduced the infection of Sclerotinia sclerotiorum and Phytophthora capsica in N. benthamiana , and overexpression of AcGF14C significantly enhanced the resistance of kiwifruit against Psa , indicating that GF14C positively regulates plant immunity. Collectively, our results revealed that the virulence effector HopZ5 could be recognized by plants and interact with GF14C to activate plant immunity. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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