Untargeted LC-HRMS applied to microcystin-producing cyanobacterial cultures for the evaluation of the efficiency of chlorine-based treatments commonly used for water potabilization.

Autor: Simonazzi M; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via Sant'Alberto 163, 48123, Ravenna, Italy; NBFC, National Biodiversity Future Center, Palermo, 90133, Italy. Electronic address: mara.simonazzi2@unibo.it., Miglione A; Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy., Tartaglione L; Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy. Electronic address: luciana.tartaglione@unina.it., Varra M; Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy., DellAversano C; Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy; NBFC, National Biodiversity Future Center, Palermo, 90133, Italy., Guerrini F; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via Sant'Alberto 163, 48123, Ravenna, Italy., Pistocchi R; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via Sant'Alberto 163, 48123, Ravenna, Italy., Pezzolesi L; Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, Via Sant'Alberto 163, 48123, Ravenna, Italy; NBFC, National Biodiversity Future Center, Palermo, 90133, Italy.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2024 Sep; Vol. 364, pp. 142976. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.chemosphere.2024.142976
Abstrakt: Cyanobacteria in water supplies are considered an emerging threat, as some species produce toxic metabolites, cyanotoxins, of which the most widespread and well-studied are microcystins. Consumption of contaminated water is a common exposure route to cyanotoxins, making the study of cyanobacteria in drinking waters a priority to protect public health. In drinking water treatment plants, pre-oxidation with chlorinated compounds is widely employed to inhibit cyanobacterial growth, although concerns on its efficacy in reducing cyanotoxin content exists. Additionally, the effects of chlorination on abundant but less-studied cyanometabolites (e.g. cyanopeptolins whose toxicity is still unclear) remain poorly investigated. Here, two chlorinated oxidants, sodium hypochlorite (NaClO) and chlorine dioxide (ClO 2 ), were tested on the toxic cyanobacterium Microcystis aeruginosa, evaluating their effect on cell viability, toxin profile and content. Intra- and extracellular microcystins and other cyanometabolites, including their degradation products, were identified using an untargeted LC-HRMS approach. Both oxidants were able to inactivate M. aeruginosa cells at a low dose (0.5 mg L -1 ), and greatly reduced intracellular toxins content (>90%), regardless of the treatment time (1-3 h). Conversely, a two-fold increase of extracellular toxins after NaClO treatment emerged, suggesting a cellular damage. A novel metabolite named cyanopeptolin-type peptide-1029, was identified based on LC-HRMS n (n = 2, 3) evidence, and it was differently affected by the two oxidants. NaClO led to increase its extracellular concentration from 2 to 80-100 μg L -1 , and ClO 2 induced the formation of its oxidized derivative, cyanopeptolin-type peptide-1045. In conclusion, pre-oxidation treatments of raw water contaminated by toxic cyanobacteria may lead to increased cyanotoxin concentrations in drinking water and, depending on the chemical agent, its dose and treatment duration, also of oxidized metabolites. Since the effects of such metabolites on human health remain unknown, this issue should be handled with extreme caution by water security agencies involved in drinking water management.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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