The Effect of Oleuropein in AIF and MMP-9 in Traumatic Brain Injury Rat Model.

Autor:	Mousa A; Department of Neurosurgery, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia, abdurrahmanmousa@usu.ac.id., Dharmajaya R; Department of Neurosurgery, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia., Reveny J; Department of Pharmacy, Pharmacy Faculty, Universitas Sumatera Utara, Medan, Indonesia., Surbakti KP; Department of Neurology, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia., Tobing HG; Department of Neurosurgery, Medical Faculty, Universitas Indonesia, Jakarta, Indonesia., Ilyas S; Department of Biology, Mathematic and Natural Sciences Faculty, Universitas, Sumatera Utara, Medan, Indonesia., Sembiring RJ; Department of Clinical Pathology, Medical Faculty, Universitas Sumatera, Utara, Medan, Indonesia., Arina CA; Department of Neurology, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia., Riawan W; Department of Biochemistry and Biology Molecular, Medical Faculty, Universitas Brawijaya, Malang, Indonesia.
Jazyk:	angličtina
Zdroj:	Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2024 Jul 31; Vol. 58 (4), pp. 361-381.
DOI:	10.33594/000000717
Abstrakt:	Background/aims: Traumatic brain injury is a significant public problem with an incidence of 10 million people per year, causing the largest deaths and disabilities worldwide. Head injuries can be classified into primary and secondary head injuries. Secondary head injuries can be caused by several factors such as ischemia, cerebral edema, and neuroinflammation. AIF and MMP-9 are two parameters that can be indicators in measuring the effect of Oleuropein on traumatic brain injury in rats. Oleuropein itself has many activities such as antioxidant, anti-apoptotic, antimicrobial, anti-inflammatory, and neuroprotective. Methods: Adult male Sprague-Dawley rats (250-350 grams) were exposed to head injury, with or without intraperitoneal administration of Oleuropein. Within 24-72 hours brain tissue was isolated for immunohistochemical analysis, ELISA, and TUNEL. AIF, GFAP, MMP-9, and HMGB-1 levels were determined using immunohistochemistry in both the control and treatment groups. Statistical analysis was made using the One-Way Analysis of Variance (ANOVA) and paired t-test. Results: The results showed that Oleuropein was able to reduce AIF and MMP-9 levels in rats with traumatic brain injury. This indicates that Oleuropein has a neuroprotective effect by reducing inflammation and apoptosis. Conclusion: Oleuropein has a potential neuroprotective effect in traumatic brain injury by reducing inflammation and apoptosis. Therefore, Oleuropein can be considered as a potential therapeutic agent for traumatic brain injury in the future. Competing Interests: The authors have nothing to disclose. (© Copyright by the Author(s). Published by Cell Physiol Biochem Press.)
Databáze:	MEDLINE
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