Ultrasound-assisted matrix solid-phase extraction based on deep eutectic solvents and zinc oxide: Extraction and determination of six active ingredients in Ligustri Lucidi Fructus.

Autor: Qian C; School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, China., Wang S; School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, China., Chen H; Institute of Technology for Chinese Medicine Preparations Industry, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, China., Li J; School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, China.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2024 Aug; Vol. 47 (15), pp. e2400275.
DOI: 10.1002/jssc.202400275
Abstrakt: In this study, we propose a novel strategy utilizing deep eutectic solvents (DESs) as both the extraction solvent and dispersing liquid, with nanometer zinc oxide (ZnO) serving as the adsorbent. This method incorporates ultrasound-assisted matrix solid phase dispersion (UA-MSPD) for the extraction of six active components (salidroside, echinacoside, acteoside, specnuezhenide, nuezhenoside G13, and oleanolic acid) from Ligustri Lucidi Fructus samples. The extracts were then analyzed using high-performance liquid chromatography equipped with a diode array detector. The effects of various parameters such as dispersant dosage, DESs volume, grinding time, ultrasonication duration, and eluent volume on extraction recovery were investigated and optimized using a central composite design under response surface methodology. The optimized conditions yielded detection limits ranging from 0.003 to 0.01 mg/g and relative standard deviations of 8.7% or lower. Extraction recoveries varied between 93% and 98%. The method demonstrated excellent linearity for the analytes (R 2 ≥ 0.9997). The simple, green, and efficient DESs/ZnO-UA-MSPD technique proved to be rapid, accurate, and reliable for extracting and analyzing the six active ingredients in Ligustri Lucidi Fructus samples.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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