Obtaining more contaminant-resistant variants from a native Chlorella vulgaris strain.

Autor: Trentini AG; Laboratorio de Biotecnología y Medio Ambiente, CEBBAD-CONICET, Universidad Maimónides, Argentina., Salvio UD; Laboratorio de Biotecnología y Medio Ambiente, CEBBAD-CONICET, Universidad Maimónides, Argentina., Sánchez Novoa JG; Laboratorio de Biotecnología y Medio Ambiente, CEBBAD-CONICET, Universidad Maimónides, Argentina., Groppa MD; IQUIFIB-CONICET, FFyB, Universidad de Buenos Aires, Argentina., Navarro Llorens JM; Metabolic Engineering Group, Department of Biochemistry and Molecular Biology, Universidad Complutense de Madrid, Madrid, Spain., Marconi PL; Laboratorio de Biotecnología y Medio Ambiente, CEBBAD-CONICET, Universidad Maimónides, Argentina. Electronic address: marconi.patricialaura@maimonides.edu.
Jazyk: angličtina
Zdroj: Revista Argentina de microbiologia [Rev Argent Microbiol] 2024 Jul-Sep; Vol. 56 (3), pp. 241-248. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.ram.2024.05.005
Abstrakt: Cildáñez stream (in Matanza-Riachuelo basin, Buenos Aires) is one of the most polluted watercourses of Argentina, containing a mixed contamination from agricultural and industrial wastes. The application of water bioremediation processes for this kind of effluent will require microorganisms with a high tolerance to contamination. In this sense, obtaining higher contaminant-resistant microalgae lines is widely desired. In this study, adaptive laboratory evolution (ALE) and random mutagenesis were used to obtain Chlorella vulgaris LMPA-40 strains adapted to grow in polluted water from the Cildáñez stream. The ALE process was performed by 22 successive subcultures under selective pressure (Cildáñez wastewater alone or with the addition of phenol or H 2 O 2 ) while random mutagenesis was performed with UV-C radiation at 275nm. Not all the cell lines obtained after ALE could adapt enough to overcome the stress caused by the Cildáñez wastewater, indicating that the process is quite random and depends on the stressor used. The best results were obtained for the Cildáñez wastewater adapted cells (Cild 3 strain) that were more resistant than the original strain. The concentration of protein, Chlorophyll A, Chlorophyll B, and carotenoids in the Cild 3 ALE evolved strain was higher than that of the control strain. However, this strain exhibited half of the lipid content compared to the same control strain. Interestingly, these alterations and the acquired tolerance may be reversed over time during storage. These findings suggest that the acquisition of novel cell lines could not be permanent, a fact that must be considered for future trials.
(Copyright © 2024 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze: MEDLINE
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