Protocol for analyzing TRAIL- and Fas-induced signaling complexes by immunoprecipitation from human cells.
| Autor: | Davidovich P; Molecular Cell Biology Laboratory, The Smurfit Institute of Genetics, Trinity College, Dublin 2, Ireland., Martin SJ; Molecular Cell Biology Laboratory, The Smurfit Institute of Genetics, Trinity College, Dublin 2, Ireland. Electronic address: martinsj@tcd.ie. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103126. Date of Electronic Publication: 2024 Jul 31. |
| DOI: | 10.1016/j.xpro.2024.103126 |
| Abstrakt: | Engagement of TRAIL or Fas death receptors can trigger the assembly of cytoplasmic caspase-8/FADD/RIPK1 (FADDosome) signaling complexes that promote nuclear factor κB (NF-κB) activation. Here, we present a protocol for immunoprecipitation of TRAIL- or Fas-induced FADDosomes from human cell lines. We describe steps for stimulating human cells with TRAIL or Fas ligand, followed by preparation of membrane death receptor-associated, as well as cytoplasmic FADDosome, signaling complexes. This protocol has application in the analysis of death receptor-induced signaling complex formation. For complete details on the use and execution of this protocol, please refer to Davidovich et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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