| Autor: |
C P H; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND., A G; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND., I G K I; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND., S V; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND., S B; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, IND. |
| Abstrakt: |
Background The fabrication of titanium carbide (Ti 3 C 2 )-cobalt sulfide (Co 3 S 4 )-based biosensors with high sensitivity and selectivity can change the biosensor manufacturing industry completely. Molecular and clinical diagnostics, disease progression monitoring, and drug discovery could utilize these sensors for early biomarker detection. MXene (Ti 3 C 2 ) is a two-dimensional material with exceptional electrical conductivity, hydrophilicity, great thermal stability, large interlayer spacing, and a high surface area. Ti 3 C 2 's remarkable characteristics make it well-suited for biomolecule immobilization and target analyte detection. Co 3 S 4 is a transition metal chalcogenide that has shown great potential in biosensors. Co 3 S 4 nanoparticles (NPs) can potentially enhance Ti 3 C 2 electrocatalytic activity, particularly in amino acid detection. L-arginine is a semi-essential amino acid, and the body frequently uses it to support healthy circulation and plays a crucial role in protein synthesis. We fabricated the Ti 3 C 2 -Co 3 S 4 biosensor for L-arginine detection. Aim This study aims to synthesize and apply Ti 3 C 2 -Co 3 S 4 nanocomposites in amino acid biosensing. Materials and methods The Ti 3 C 2 nanosheets were synthesized by the selective removal of an aluminum (Al) layer from the precursor (Ti 3 AlC 2 ) using hydrofluoric acid (HF). The resulting mixture serves as an etchant, especially targeting the Al layers on Ti 3 AlC 2 while protecting the desired MXene layers at room temperature. Cobalt nitrate hexahydrate was dissolved in deionized water. Sodium hydroxide was added to the cobalt solution and stirred. Thioacetamide was added to the above solution and stirred (Solution B). A mixture of Solution A and Solution B was stirred for 30 minutes. The mixture is transferred to a hydrothermal reactor and maintained at a temperature of 180°C for 12 hours. Once the reaction completes, we cool the resultant mixture to room temperature and then filter it using the washing technique. The sample underwent a 12-hour drying process at 80°C. Results This study investigated the use of a biosensor that employed Ti 3 C 2 -Co 3 S 4 NPs to detect the concentration of L-arginine. The X-ray diffraction (XRD) shows clear and distinct peaks, which means that the synthesized Ti 3 C 2 -Co 3 S 4 nanostructures have a crystalline structure. Scanning electron microscopy (SEM) analysis revealed that the sheetlike structure of synthesized Ti 3 C 2 -Co 3 S 4 nanostructures revealed the crystalline morphology. The results of this study show that the Ti 3 C 2 -Co 3 S 4 NP-based biosensor can be used to detect L-arginine in a sensitive and selective way. Conclusion This study investigated the synthesis of Ti 3 C 2 -Co 3 S 4 NPs and their ability to detect L-arginine levels and show a distinct correlation between the L-arginine concentration and the fluorescence intensity, demonstrating the biosensor's effectiveness in detecting L-arginine levels.
Competing Interests: Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work.
(Copyright © 2024, C P et al.) |
