Oral specimens as a tool for accurate metagenomic analysis: A pilot study.

Autor: Rodríguez-Fuentes ME; Faculty of Medicine and Dentistry, Universidade de Santiago de Compostela, c/ Entrerríos s/n, Santiago de Compostela 15782, Spain; Health Research Institute of Santiago de Compostela (IDIS, ORALRES Group), Santiago de Compostela, A Coruña 15706, Spain., Pérez-Sayáns M; Faculty of Medicine and Dentistry, Universidade de Santiago de Compostela, c/ Entrerríos s/n, Santiago de Compostela 15782, Spain; Health Research Institute of Santiago de Compostela (IDIS, ORALRES Group), Santiago de Compostela, A Coruña 15706, Spain. Electronic address: mario.perez@usc.es., Barbeito-Castiñeiras G; Microbiology Unit, Complexo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, A Coruña 15706, Spain., Molares-Vila A; Health Research Institute of Santiago de Compostela (IDIS, ORALRES Group), Santiago de Compostela, A Coruña 15706, Spain; Health Research Institute of Santiago de Compostela (IDIS, RESMET Group, https://resmet.org), Santiago de Compostela, A Coruña 15706, Spain., Prado-Pena IB; Faculty of Medicine and Dentistry, Universidade de Santiago de Compostela, c/ Entrerríos s/n, Santiago de Compostela 15782, Spain., Camolesi GCV; Faculty of Medicine and Dentistry, Universidade de Santiago de Compostela, c/ Entrerríos s/n, Santiago de Compostela 15782, Spain; Health Research Institute of Santiago de Compostela (IDIS, ORALRES Group), Santiago de Compostela, A Coruña 15706, Spain., López-López R; Faculty of Medicine and Dentistry, Universidade de Santiago de Compostela, c/ Entrerríos s/n, Santiago de Compostela 15782, Spain; Medical Oncology Unit, Complexo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, A Coruña 15706, Spain.
Jazyk: angličtina
Zdroj: Journal of stomatology, oral and maxillofacial surgery [J Stomatol Oral Maxillofac Surg] 2024 Oct; Vol. 125 (5S2), pp. 101991. Date of Electronic Publication: 2024 Jul 30.
DOI: 10.1016/j.jormas.2024.101991
Abstrakt: Objectives: Acute oral mucosal damage, as well as other inflammatory processes seem to be related to dysbiosis of the oral microbiome. The need to study changes in the oral microbiome led us to hypothesize what type of sample would provide the most representative picture of the entire human oral microbiome.
Materials and Methods: An observational, and cross-sectional study was carried out. Six healthy adult participants provided 3 different sample types each, that included saliva, oral rinse and mucosal biopsy tissue. We performed 16S rRNA sequencing of the V3-V4 region of the 18 samples using Illumina MiSeq technology.
Results: Participants were 27 ± 6,3 years old. Bacterial alpha diversity was higher in oral rinse samples compared to whole unstimulated saliva and oral mucosa tissue (p = 0,005). However, saliva specimens showed a 56 % relative abundance of identified species followed by a 30 % in oral rinse and only 1 % in tissue samples.
Conclusions: This study found differences on oral microbiome composition for each type of sample. Oral rinse should be chosen when higher alpha diversity is needed, whereas whole unstimulated saliva should be more appropriate for larger amount of bacterial DNA.
Clinical Relevance: The results obtained demonstrate the importance of a correct choice of the optimal type of oral sample for microbiome studies due to the differences found in its composition.
Competing Interests: Declaration of competing interest None
(Copyright © 2024 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: