A Detailed Re-Examination of the Period Gene Rescue Experiments Shows That Four to Six Cryptochrome-Positive Posterior Dorsal Clock Neurons (DN 1p ) of Drosophila melanogaster Can Control Morning and Evening Activity.

Autor: Sekiguchi M; Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan., Reinhard N; Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany., Fukuda A; Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan., Katoh S; Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, Okayama, Japan., Rieger D; Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany., Helfrich-Förster C; Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, Würzburg, Germany., Yoshii T; Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.; Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, Okayama, Japan.
Jazyk: angličtina
Zdroj: Journal of biological rhythms [J Biol Rhythms] 2024 Oct; Vol. 39 (5), pp. 463-483. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1177/07487304241263130
Abstrakt: Animal circadian clocks play a crucial role in regulating behavioral adaptations to daily environmental changes. The fruit fly Drosophila melanogaster exhibits 2 prominent peaks of activity in the morning and evening, known as morning (M) and evening (E) peaks. These peaks are controlled by 2 distinct circadian oscillators located in separate groups of clock neurons in the brain. To investigate the clock neurons responsible for the M and E peaks, a cell-specific gene expression system, the GAL4-UAS system, has been commonly employed. In this study, we re-examined the two-oscillator model for the M and E peaks of Drosophila by utilizing more than 50 Gal4 lines in conjunction with the UAS-period 16 line, which enables the restoration of the clock function in specific cells in the period ( per ) null mutant background. Previous studies have indicated that the group of small ventrolateral neurons (s-LN v ) is responsible for controlling the M peak, while the other group, consisting of the 5 th ventrolateral neuron (5 th LN v ) and the three cryptochrome (CRY)-positive dorsolateral neurons (LN d ), is responsible for the E peak. Furthermore, the group of posterior dorsal neurons 1 (DN 1p ) is thought to also contain M and E oscillators. In this study, we found that Gal4 lines directed at the same clock neuron groups can lead to different results, underscoring the fact that activity patterns are influenced by many factors. Nevertheless, we were able to confirm previous findings that the entire network of circadian clock neurons controls M and E peaks, with the lateral neurons playing a dominant role. In addition, we demonstrate that 4 to 6 CRY-positive DN 1p cells are sufficient to generate M and E peaks in light-dark cycles and complex free-running rhythms in constant darkness. Ultimately, our detailed screening could serve as a catalog to choose the best Gal4 lines that can be used to rescue per in specific clock neurons.
Competing Interests: Conflict of interest statementThe authors have no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze: MEDLINE
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