Combined Flow-Fluorescence in situ hybridization to HHV-8 and EBV reveals the viral heterogeneity of primary effusion lymphoma.

Autor:	Stammler R; Inserm U976 HIPI, Institut Universitaire d'Hématologie, Université Paris Cité, Paris, France., Vacher L; Department of Clinical Immunology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Fournier B; Laboratory of Lymphocyte Activation and Susceptibility to EBV infection, INSERM UMR 1163, Imagine Institute, Université Paris Cité, Paris, France., Lemaire P; Laboratory of Haematology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Chauvel C; Laboratory of Haematology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Silvestrini MA; Inserm U976 HIPI, Institut Universitaire d'Hématologie, Université Paris Cité, Paris, France.; Laboratory of Lymphocyte Activation and Susceptibility to EBV infection, INSERM UMR 1163, Imagine Institute, Université Paris Cité, Paris, France., Knapp S; Inserm U976 HIPI, Institut Universitaire d'Hématologie, Université Paris Cité, Paris, France., de Frémont GM; Inserm U976 HIPI, Institut Universitaire d'Hématologie, Université Paris Cité, Paris, France., Meignin V; National Reference Center for Castleman disease, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.; Pathology Department, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Salmona M; Laboratory of Virology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Legoff J; Laboratory of Virology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Vanjak A; Inserm U976 HIPI, Institut Universitaire d'Hématologie, Université Paris Cité, Paris, France., Dunogué B; Department of Internal Medicine, Hôpital Cochin, AP-HP, Université Paris Cité, Paris, France., Urbain F; Department of Internal Medicine, Hôpital Bicêtre, AP-HP, Le Kremlin-Bicêtre, Université Paris Saclay, Le Kremlin-Bicetre, France., Lambotte O; Department of Internal Medicine, Hôpital Bicêtre, AP-HP, Le Kremlin-Bicêtre, Université Paris Saclay, Le Kremlin-Bicetre, France., Noël N; Department of Internal Medicine, Hôpital Bicêtre, AP-HP, Le Kremlin-Bicêtre, Université Paris Saclay, Le Kremlin-Bicetre, France., Gérard L; Department of Clinical Immunology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.; National Reference Center for Castleman disease, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Oksenhendler E; Department of Clinical Immunology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.; National Reference Center for Castleman disease, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Galicier L; Department of Clinical Immunology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.; National Reference Center for Castleman disease, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France., Latour S; Laboratory of Lymphocyte Activation and Susceptibility to EBV infection, INSERM UMR 1163, Imagine Institute, Université Paris Cité, Paris, France., Boutboul D; Inserm U976 HIPI, Institut Universitaire d'Hématologie, Université Paris Cité, Paris, France.; Laboratory of Lymphocyte Activation and Susceptibility to EBV infection, INSERM UMR 1163, Imagine Institute, Université Paris Cité, Paris, France.; National Reference Center for Castleman disease, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.; Department of Hematology, Hôpital Cochin, AP-HP, Université Paris Cité, Paris, France.
Jazyk:	angličtina
Zdroj:	Journal of medical virology [J Med Virol] 2024 Aug; Vol. 96 (8), pp. e29836.
DOI:	10.1002/jmv.29836
Abstrakt:	Primary effusion lymphoma (PEL) is a rare B-cell non-Hodgkin lymphoma associated with Kaposi Sarcoma-associated herpesvirus (KSHV/HHV8) infection. Lymphoma cells are coinfected with Epstein-Barr virus (EBV) in 60-80% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports PEL diagnosis in 4 patients using a Flow-Fluorescence in situ hybridization (FlowFISH) technique that allowed detection of differentially expressed EBV and HHV8 transcripts within the same sample, revealing viral heterogeneity of the disease. Moreover, infected cells exhibited variable expressions of CD19, CD38, CD40, and CD138. Therefore, FlowFISH is a promising tool to diagnose and characterize complex viral lymphoproliferations. (© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.)
Databáze:	MEDLINE
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