A fully sequenced collection of homozygous EMS mutants for forward and reverse genetic screens in Arabidopsis thaliana.

Autor: Carrère S; LIPME, Université de Toulouse, INRAE/CNRS UMR 0441/2598, Castanet-Tolosan, France., Routaboul JM; LIPME, Université de Toulouse, INRAE/CNRS UMR 0441/2598, Castanet-Tolosan, France., Savourat P; Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin for Plant Sciences (IJPB), 78000, Versailles, France., Bellenot C; LIPME, Université de Toulouse, INRAE/CNRS UMR 0441/2598, Castanet-Tolosan, France., López H; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Cologne, Germany., Sahoo A; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Cologne, Germany., Quiroz Monnens T; LIPME, Université de Toulouse, INRAE/CNRS UMR 0441/2598, Castanet-Tolosan, France., Ricou A; Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin for Plant Sciences (IJPB), 78000, Versailles, France., Camilleri C; Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin for Plant Sciences (IJPB), 78000, Versailles, France., Declerck N; CBS, Université Montpellier, CNRS/INSERM, UMR5048/1054, Montpellier, France., Laufs P; Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin for Plant Sciences (IJPB), 78000, Versailles, France., Mercier R; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Cologne, Germany., Noël LD; LIPME, Université de Toulouse, INRAE/CNRS UMR 0441/2598, Castanet-Tolosan, France.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Jul 29. Date of Electronic Publication: 2024 Jul 29.
DOI: 10.1111/tpj.16954
Abstrakt: Genetic screens are powerful tools for biological research and are one of the reasons for the success of the thale cress Arabidopsis thaliana as a research model. Here, we describe the whole-genome sequencing of 871 Arabidopsis lines from the Homozygous EMS Mutant (HEM) collection as a novel resource for forward and reverse genetics. With an average 576 high-confidence mutations per HEM line, over three independent mutations altering protein sequences are found on average per gene in the collection. Pilot reverse genetics experiments on reproductive, developmental, immune and physiological traits confirmed the efficacy of the tool for identifying both null, knockdown and gain-of-function alleles. The possibility of conducting subtle repeated phenotyping and the immediate availability of the mutations will empower forward genetic approaches. The sequence resource is searchable with the ATHEM web interface (https://lipm-browsers.toulouse.inra.fr/pub/ATHEM/), and the biological material is distributed by the Versailles Arabidopsis Stock Center.
(© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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