Genomic Insights of a Methicillin-Resistant Biofilm-Producing Staphylococcus aureus Strain Isolated From Food Handlers.

Autor: Ballah FM; Department of Microbiology and Hygiene Faculty of Veterinary Science Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Hoque MN; Molecular Biology and Bioinformatics Laboratory Department of Gynaecology Obstetrics and Reproductive Health Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh., Islam MS; Department of Microbiology and Hygiene Faculty of Veterinary Science Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.; Department of Animal Sciences University of California-Davis, Davis, California CA 95616, USA., Faisal GM; Molecular Biology and Bioinformatics Laboratory Department of Gynaecology Obstetrics and Reproductive Health Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh., Rahman AMT; Department of Medicine Rajshahi Medical College, Rajshahi 6100, Bangladesh., Khatun MM; Department of Microbiology and Hygiene Faculty of Veterinary Science Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Rahman M; Department of Microbiology and Hygiene Faculty of Veterinary Science Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Hassan J; Department of Microbiology and Hygiene Faculty of Veterinary Science Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Rahman MT; Department of Microbiology and Hygiene Faculty of Veterinary Science Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2024 Jul 20; Vol. 2024, pp. 5516117. Date of Electronic Publication: 2024 Jul 20 (Print Publication: 2024).
DOI: 10.1155/2024/5516117
Abstrakt: Methicillin-resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of a strong biofilm-producing and multidrug-resistant (MDR) S. aureus MTR_BAU_H1 strain through whole-genome sequencing (WGS). The S. aureus MTR_BAU_H1 strain was isolated from food handlers' hand swabs in Bangladesh and phenotypically assessed for antimicrobial susceptibility and biofilm production assays. The isolate was further undergone to high throughput WGS and analysed using different bioinformatics tools to elucidate the genetic diversity, molecular epidemiology, sequence type (ST), antimicrobial resistance, and virulence gene distribution. Phenotypic analyses revealed that the S. aureus MTR_BAU_H1 strain is a strong biofilm-former and carries both antimicrobial resistance (e.g., methicillin resistance; mec A, beta-lactam resistance; bla Z and tetracycline resistance; tet C) and virulence (e.g., sea , tsst , and PVL ) genes. The genome of the S. aureus MTR_BAU_H1 belonged to ST1930 that possessed three plasmid replicons (e.g., rep16, rep7c, and rep19), seven prophages, and two clustered regularly interspaced short palindromic repeat (CRISPR) arrays of varying sizes. Phylogenetic analysis showed a close evolutionary relationship between the MTR_BAU_H1 genome and other MRSA clones of diverse hosts and demographics. The MTR_BAU_H1 genome harbours 42 antimicrobial resistance genes (ARGs), 128 virulence genes, and 273 SEED subsystems coding for the metabolism of amino acids, carbohydrates, proteins, cofactors, vitamins, minerals, and lipids. This is the first-ever WGS-based study of a strong biofilm-producing and MDR S. aureus strain isolated from human hand swabs in Bangladesh that unveils new information on the resistomes (ARGs and correlated mechanisms) and virulence potentials that might be linked to staphylococcal pathogenesis in both humans and animals.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2024 Fatimah Muhammad Ballah et al.)
Databáze: MEDLINE
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