Protective effects of mesenchymal stromal cell-derived secretome on dermonecrosis induced in rabbits by Loxosceles intermedia spider venom.

Autor:	Rodrigues GM; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., de Almeida ME; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Marcelino SAC; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Fernandes PBU; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., da Cruz JOP; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Araújo FL; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Ferreira RDS; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Botelho AFM; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Bedoya FJ; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain.; Biomedical Research Network for Diabetes and Related Metabolic Diseases (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain., Cahuana GM; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain.; Biomedical Research Network for Diabetes and Related Metabolic Diseases (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain., Hitos AB; Institute of Bioengineering and Institute of Biomedical Research ISABIAL, University Miguel Hernández de Elche, Alicante, Spain., Soria B; Biomedical Research Network for Diabetes and Related Metabolic Diseases (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain.; Institute of Bioengineering and Institute of Biomedical Research ISABIAL, University Miguel Hernández de Elche, Alicante, Spain., Costal-Oliveira F; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Duarte CG; Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil., Tejedo JR; Department of Molecular Biology and Biochemical Engineering, Universidad Pablo de Olavide, Seville, Spain.; Biomedical Research Network for Diabetes and Related Metabolic Diseases (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain.; Institute of Tropical Diseases, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Chachapoyas, Peru., Chávez-Olórtegui C; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., Melo MM; Department of Veterinary Clinic and Surgery, Veterinary College, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
Jazyk:	angličtina
Zdroj:	The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2024 Jul 22; Vol. 30, pp. e20240004. Date of Electronic Publication: 2024 Jul 22 (Print Publication: 2024).
DOI:	10.1590/1678-9199-JVATITD-2024-0004
Abstrakt:	Background: Loxoscelism refers to a set of clinical manifestations caused by the bite of spiders from the Loxosceles genus. The classic clinical symptoms are characterized by an intense inflammatory reaction at the bite site followed by local necrosis and can be classified as cutaneous loxoscelism. This cutaneous form presents difficult healing, and the proposed treatments are not specific or effective. This study aimed to evaluate the protective effect of mesenchymal stromal cells-derived secretome on dermonecrosis induced by Loxosceles intermedia spider venom in rabbits. Methods: Sixteen rabbits were distributed into four groups (n = 4). Except for group 1 (G1), which received only PBS, the other three groups (G2, G3, and G4) were initially challenged with 10 μg of L. intermedia venom, diluted in 100 μL of NaCl 0.9%, by intradermic injection in the interscapular region. Thirty minutes after the challenge all groups were treated with secretome, except for group 2. Group 1 (G1-control group) received intradermal injection (ID) of 60 μg of secretome in 0.15 M PBS; Group 2 (G2) received 0.9% NaCl via ID; Group 3 (G3) received 60 μg of secretome, via ID and Group 4 (G4), received 60 μg of secretome by intravenous route. Rabbits were evaluated daily and after 15 days were euthanized, necropsied and skin samples around the necrotic lesions were collected for histological analysis. Results: Rabbits of G1 did not present edema, erythema, hemorrhagic halo, or necrosis. In animals from G2, G3, and G4, edema appeared after 6h. However, minor edema was observed in the animals of G2 and G3. Hemorrhagic halo was observed in animals, six hours and three days after, on G2, G3, and G4. Macroscopically, in G4, only one animal out of four had a lesion that evolved into a dermonecrotic wound. No changes were observed in the skin of the animals of G1, by microscopic evaluation. All animals challenged with L. intermedia venom showed similar alterations, such as necrosis and heterophilic infiltration. However, animals from G4 showed fibroblast activation, early development of connective tissue, neovascularization, and tissue re-epithelialization, indicating a more prominent healing process. Conclusion: These results suggest that secretome from mesenchymal stromal cells cultured in a xeno-free and human component-free culture media can be promising to treat dermonecrosis caused after Loxosceles spiders bite envenoming. Competing Interests: Competing interest : The authors declare that they have no conflict of interest.
Databáze:	MEDLINE
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