Anti-proliferative impact of resveratrol on gingival fibroblasts from juvenile hyaline fibromatosis.

Autor: Saygun I; Department of Periodontology, Gulhane Faculty of Dental Medicine, University of Health Sciences, Ankara, Turkey. saygunisil@hotmail.com., Slezovic MÖ; Department of Periodontology, Gulhane Faculty of Dental Medicine, University of Health Sciences, Ankara, Turkey. melis.ozgulslezovic@sbu.edu.tr., Özkan CK; Gulhane Faculty of Pharmacy, University of Health Sciences, Ankara, Turkey., Bengi VU; Department of Periodontology, Gulhane Faculty of Dental Medicine, University of Health Sciences, Ankara, Turkey., Elçi P; Gulhane Health Sciences Institute, Stem Cell Lab, University of Health Sciences, Ankara, Turkey., Serdar M; Department of Medical Biochemistry, Acıbadem Mehmet Ali Aydınlar University, Ankara, Turkey., Kantarci A; Forsyth Institute, Cambridge, MA, USA.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2024 Jul 26; Vol. 28 (8), pp. 448. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1007/s00784-024-05771-7
Abstrakt: Aim: Resveratrol is a natural polyphenolic compound with biological activities such as anti-inflammation and antioxidation. Its anti-fibrotic effect has been experimentally demonstrated in the pancreas and liver. This study aims to determine the anti-proliferative effect of resveratrol on fibroblasts obtained from hyperplastic gingival tissues from a patient diagnosed with Juvenile Hyaline Fibromatosis (JHF).
Materials and Methods: Primary gingival fibroblast cell lines were obtained from gingival growth tissues by the gingivectomy of a patient with JHF. Gingival fibroblasts were treated with or without 3 different doses of resveratrol (50, 100, 200 µM). Cytotoxicity and cell proliferation were evaluated after 24, 48, and 72 h. Collagen, TGF, and CTGF were analyzed by ELISA in the 48-hour supernatants.
Results: All three doses of resveratrol suppressed the proliferation of JHF gingival fibroblasts at 24 and 48 h without showing any cytotoxic effect compared to the control group (p < 0.0001). At 72 h, 100 and 200 µM resveratrol showed significantly less proliferation (p < 0.0001), less collagen, CTGF, and TGF- β (p < 0.001) than the control group.
Conclusion: Resveratrol had a profound anti-proliferative effect on gingival fibroblasts obtained from gingival enlargements with JHF, suggesting that it can be used as a therapeutic to prevent excessive cell growth by suppressing collagen, CTGF, and TGF- β synthesis in the pathogenesis of hyperplasia.
(© 2024. The Author(s).)
Databáze: MEDLINE
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