Exploring protein structural adaptations and polyphenol interactions: Influences on digestibility in pigeon pea dal and whole grains under heat and germination conditions.

Autor: Dutta M; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Dineshkumar R; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Nagesh CR; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Durga Lakshmi Y; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Lekhak B; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Bansal N; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Goswami S; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Kumar RR; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Kundu A; Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Mandal PK; National Institute for Plant Biotechnology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Arora B; Division of Food Science & Post Harvest Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Raje RS; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Mandal S; Department of Biochemistry, Uttar Banga Krishi Viswavidyalaya, Coochbehar, West Bengal, India., Yadav A; Division of Agricultural Physics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Tyagi A; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India., Ramesh SV; ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, 671 124, India., Rama Prashat G; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: rama.g@icar.gov.in., Vinutha T; Division of Biochemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: vinutha.t@icar.gov.in.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Dec 01; Vol. 460 (Pt 1), pp. 140561. Date of Electronic Publication: 2024 Jul 20.
DOI: 10.1016/j.foodchem.2024.140561
Abstrakt: Pigeon pea, a protein-rich legume with low protein digestibility (PD) due to its high polyphenol content and other antinutritional factors (ANFs). Consequently, processing methods are crucial to improve PD. We investigated the effects of thermal treatments (cooking, hydrothermal, autoclaving, infrared rays) treatments and germination on modulation of PD, its properties and association with ANFs in two distinct genotypes based on polyphenol content: high (Pusa Arhar 2018-4) and low (ICP-1452). Treatments improved in vitro PD and essential amino acid content, with autoclaving showing significantly higher PD (ICP-1452: 90.4%, Pusa-Arhar 2018-4: 84.32%) ascribed to disruption of tight protein matrices. Significant increase in β-turn, reduction in protein: starch, protein: polyphenol interactions as well as breakdown of storage proteins revealed by the analysis of protein structural properties. This study suggests thermal treatments, particularly autoclaving, can enhance pigeon pea protein's nutritional quality for its utilization as a new ingredient in development of healthy foods.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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